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Literature DB >> 17575049

Positive feedback between Dia1, LARG, and RhoA regulates cell morphology and invasion.

Thomas M Kitzing¹, Arul S Sahadevan, Dominique T Brandt, Helga Knieling, Sebastian Hannemann, Oliver T Fackler, Jörg Grosshans, Robert Grosse.

Abstract

The RhoA-effector Dia1 controls actin-dependent processes such as cytokinesis, SRF transcriptional activity, and cell motility. Dia1 polymerizes actin through its formin homology (FH) 2 domain. Here we show that Dia1 acts upstream of RhoA independently of its effects on actin assembly. Dia1 binds to the leukemia-associated Rho-GEF (LARG) through RhoA-dependent release of Dia1 autoinhibition. The FH2 domain stimulates the guanine nucleotide exchange activity of LARG in vitro. Our results reveal that Dia1 is necessary for LPA-stimulated Rho/ROCK signaling and bleb-associated cancer cell invasion. Thus, Dia1-dependent RhoA activation constitutes a positive feedback mechanism to modulate cell behavior.

Entities: Chemical Disease Gene

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Year: 2007 PMID： 17575049 PMCID： PMC1891425 DOI： 10.1101/gad.424807

Source DB: PubMed Journal: Genes Dev ISSN： 0890-9369 Impact factor: 11.361

32 in total

1. Lineage infidelity of MDA-MB-435 cells: expression of melanocyte proteins in a breast cancer cell line.

Authors: Shankar Sellappan; Rebecca Grijalva; Xiaoyan Zhou; Wentao Yang; Menashe Bar Eli; Gordon B Mills; Dihua Yu
Journal: Cancer Res Date: 2004-05-15 Impact factor: 12.701

2. Crystal structures of a Formin Homology-2 domain reveal a tethered dimer architecture.

Authors: Yingwu Xu; James B Moseley; Isabelle Sagot; Florence Poy; David Pellman; Bruce L Goode; Michael J Eck
Journal: Cell Date: 2004-03-05 Impact factor: 41.582

3. The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization.

Authors: Atsushi Shimada; Miklós Nyitrai; Ingrid R Vetter; Dorothee Kühlmann; Beáta Bugyi; Shuh Narumiya; Michael A Geeves; Alfred Wittinghofer
Journal: Mol Cell Date: 2004-02-27 Impact factor: 17.970

4. Structural basis of actin filament nucleation and processive capping by a formin homology 2 domain.

Authors: Takanori Otomo; Diana R Tomchick; Chinatsu Otomo; Sanjay C Panchal; Mischa Machius; Michael K Rosen
Journal: Nature Date: 2005-01-05 Impact factor: 49.962

5. Modular architecture and novel protein-protein interactions regulating the RGS-containing Rho guanine nucleotide exchange factors.

Authors: José Vázquez-Prado; John Basile; J Silvio Gutkind
Journal: Methods Enzymol Date: 2004 Impact factor: 1.600

Review 6. Mechanisms of cancer cell invasion.

Authors: Erik Sahai
Journal: Curr Opin Genet Dev Date: 2005-02 Impact factor: 5.578

7. Signal-regulated activation of serum response factor is mediated by changes in actin dynamics.

Authors: A Sotiropoulos; D Gineitis; J Copeland; R Treisman
Journal: Cell Date: 1999-07-23 Impact factor: 41.582

8. Regulation of RGS-RhoGEFs by Galpha12 and Galpha13 proteins.

Authors: Shihori Tanabe; Barry Kreutz; Nobuchika Suzuki; Tohru Kozasa
Journal: Methods Enzymol Date: 2004 Impact factor: 1.600

9. Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysis.

Authors: Erik Sahai; Christopher J Marshall
Journal: Nat Cell Biol Date: 2003-08 Impact factor: 28.824

10. Homo- and hetero-oligomerization of PDZ-RhoGEF, LARG and p115RhoGEF by their C-terminal region regulates their in vivo Rho GEF activity and transforming potential.

Authors: Hiroki Chikumi; Ana Barac; Babak Behbahani; Yuan Gao; Hidemi Teramoto; Yi Zheng; J Silvio Gutkind
Journal: Oncogene Date: 2004-01-08 Impact factor: 9.867

77 in total

1. Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation.

Authors: Jana Stastna; Xiaoyu Pan; Haicui Wang; Alina Kollmannsperger; Stefan Kutscheidt; Volker Lohmann; Robert Grosse; Oliver T Fackler
Journal: Cell Res Date: 2011-12-20 Impact factor: 25.617

Review 2. Understanding cytokinesis failure.

Authors: Guillaume Normand; Randall W King
Journal: Adv Exp Med Biol Date: 2010 Impact factor: 2.622

Positive feedback between Dia1, LARG, and RhoA regulates cell morphology and invasion.

1. Lineage infidelity of MDA-MB-435 cells: expression of melanocyte proteins in a breast cancer cell line.

2. Crystal structures of a Formin Homology-2 domain reveal a tethered dimer architecture.

3. The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization.

4. Structural basis of actin filament nucleation and processive capping by a formin homology 2 domain.

5. Modular architecture and novel protein-protein interactions regulating the RGS-containing Rho guanine nucleotide exchange factors.

Review 6. Mechanisms of cancer cell invasion.

7. Signal-regulated activation of serum response factor is mediated by changes in actin dynamics.

8. Regulation of RGS-RhoGEFs by Galpha12 and Galpha13 proteins.

9. Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysis.

10. Homo- and hetero-oligomerization of PDZ-RhoGEF, LARG and p115RhoGEF by their C-terminal region regulates their in vivo Rho GEF activity and transforming potential.

1. Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation.

Review 2. Understanding cytokinesis failure.

3. DRhoGEF2 and diaphanous regulate contractile force during segmental groove morphogenesis in the Drosophila embryo.

4. Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells.

Review 5. Role of G12 proteins in oncogenesis and metastasis.

Review 6. Regulation and physiological functions of G12/13-mediated signaling pathways.

7. Dynamic regulation of ROCK in tumor cells controls CXCR4-driven adhesion events.

8. Overexpression of FMNL2 is closely related to metastasis of colorectal cancer.

9. Xenopus Paraxial Protocadherin regulates morphogenesis by antagonizing Sprouty.

10. MiR-126 suppresses colon cancer cell proliferation and invasion via inhibiting RhoA/ROCK signaling pathway.