Literature DB >> 17572846

Easy stable transfection of a human cancer cell line by electrogene transfer with an Epstein-Barr virus-based plasmid vector.

Masa-Aki Shibata1, Yoshihiro Miwa, Junji Morimoto, Yoshinori Otsuki.   

Abstract

We report an easy and stable transfection technique using electrogene transfer with a nonviral Epstein-Barr (EB) virus-based vector. To achieve stable transfection of human breast cancer cells, we conducted electrogene transfer of an EB virus-based plasmid vector (reduced size of oriP) containing the enhanced green fluorescence protein (eGFP) gene. Because the EB virus-based vector exhibits high transfer efficiency and strong persistent transgene expression as a result of autonomous replication in human cells, and as Nucleofector electrogene transfer can achieve highly efficient gene transfection, this method is particularly suitable for generation of stably transfected cell lines.

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Year:  2007        PMID： 17572846     DOI： 10.1007/s00795-007-0358-7

Source DB:  PubMed          Journal:  Med Mol Morphol        ISSN： 1860-1499            Impact factor:   2.309


  12 in total

1.  Effective suicide gene therapy in vivo by EBV-based plasmid vector coupled with polyamidoamine dendrimer.

Authors:  H Maruyama-Tabata; Y Harada; T Matsumura; E Satoh; F Cui; M Iwai; M Kita; S Hibi; J Imanishi; T Sawada; O Mazda
Journal:  Gene Ther       Date:  2000-01       Impact factor: 5.250

2.  Enhanced expression of recombinant dystrophin following intramuscular injection of Epstein-Barr virus (EBV)-based mini-chromosome vectors in mdx mice.

Authors:  H Tsukamoto; D Wells; S Brown; P Serpente; P Strong; J Drew; K Inui; S Okada; G Dickson
Journal:  Gene Ther       Date:  1999-07       Impact factor: 5.250

3.  Polyethylenimine-mediated suicide gene transfer induces a therapeutic effect for hepatocellular carcinoma in vivo by using an Epstein-Barr virus-based plasmid vector.

Authors:  Masaki Iwai; Yoshinori Harada; Saiyu Tanaka; Akira Muramatsu; Takahiro Mori; Kei Kashima; Jiro Imanishi; Osam Mazda
Journal:  Biochem Biophys Res Commun       Date:  2002-02-15       Impact factor: 3.575

4.  Direct intra-cardiomuscular transfer of beta2-adrenergic receptor gene augments cardiac output in cardiomyopathic hamsters.

Authors:  K Tomiyasu; Y Oda; M Nomura; E Satoh; S Fushiki; J Imanishi; M Kondo; O Mazda
Journal:  Gene Ther       Date:  2000-12       Impact factor: 5.250

5.  EBV replicon vector system enhances transgene expression in vivo: applications to cancer gene therapy.

Authors:  T Otomo; S Yamamoto; R Morishita; Y Kaneda
Journal:  J Gene Med       Date:  2001 Jul-Aug       Impact factor: 4.565

6.  Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells.

Authors:  J L Yates; N Warren; B Sugden
Journal:  Nature       Date:  1985 Feb 28-Mar 6       Impact factor: 49.962

7.  Electrogene therapy using endostatin, with or without suicide gene therapy, suppresses murine mammary tumor growth and metastasis.

Authors:  M-A Shibata; J Morimoto; H Doi; S Morishima; M Naka; Y Otsuki
Journal:  Cancer Gene Ther       Date:  2006-11-10       Impact factor: 5.987

8.  Intravascular insulin gene delivery as potential therapeutic intervention in diabetes mellitus.

Authors:  Kakei Yasutomi; Yoshiki Itokawa; Hidetsugu Asada; Tsunao Kishida; Feng-De Cui; Suzuyo Ohashi; Satoshi Gojo; Yuji Ueda; Toshikazu Kubo; Hisakazu Yamagishi; Jiro Imanishi; Toshiyuki Takeuchi; Osam Mazda
Journal:  Biochem Biophys Res Commun       Date:  2003-10-24       Impact factor: 3.575

9.  Successful transfer of ADA gene in vitro into human peripheral blood CD34+ cells by transfecting EBV-based episomal vectors.

Authors:  E Satoh; H Hirai; T Inaba; C Shimazaki; M Nakagawa; J Imanishi; O Mazda
Journal:  FEBS Lett       Date:  1998-12-11       Impact factor: 4.124

Review 10.  Improvement of nonviral gene therapy by Epstein-Barr virus (EBV)-based plasmid vectors.

Authors:  O Mazda
Journal:  Curr Gene Ther       Date:  2002-09       Impact factor: 4.391
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  4 in total

1.  Dual gene expression in embryoid bodies derived from human induced pluripotent stem cells using episomal vectors.

Authors:  Minoru Tomizawa; Fuminobu Shinozaki; Yasufumi Motoyoshi; Takao Sugiyama; Shigenori Yamamoto; Makoto Sueishi
Journal:  Tissue Eng Part A       Date:  2014-12       Impact factor: 3.845

2.  Expression of GABAergic system in pulmonary neuroendocrine cells and airway epithelial cells in GAD67-GFP knock-in mice.

Authors:  Yasuaki Yabumoto; Masahito Watanabe; Yuko Ito; Kentaro Maemura; Yoshinori Otsuki; Yumi Nakamura; Yuchio Yanagawa; Kunihiko Obata; Katsuya Watanabe
Journal:  Med Mol Morphol       Date:  2008-05-11       Impact factor: 2.309

3.  α-Mangostin extracted from the pericarp of the mangosteen (Garcinia mangostana Linn) reduces tumor growth and lymph node metastasis in an immunocompetent xenograft model of metastatic mammary cancer carrying a p53 mutation.

Authors:  Masa-Aki Shibata; Munekazu Iinuma; Junji Morimoto; Hitomi Kurose; Kanako Akamatsu; Yasushi Okuno; Yukihiro Akao; Yoshinori Otsuki
Journal:  BMC Med       Date:  2011-06-03       Impact factor: 8.775

4.  Alterations in cell cycle and induction of apoptotic cell death in breast cancer cells treated with α-mangostin extracted from mangosteen pericarp.

Authors:  Hitomi Kurose; Masa-Aki Shibata; Munekazu Iinuma; Yoshinori Otsuki
Journal:  J Biomed Biotechnol       Date:  2012-04-08
  4 in total

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