| Literature DB >> 14550289 |
Kakei Yasutomi1, Yoshiki Itokawa, Hidetsugu Asada, Tsunao Kishida, Feng-De Cui, Suzuyo Ohashi, Satoshi Gojo, Yuji Ueda, Toshikazu Kubo, Hisakazu Yamagishi, Jiro Imanishi, Toshiyuki Takeuchi, Osam Mazda.
Abstract
We assessed therapeutic potential of intravascular insulin gene delivery in a diabetic murine model. The rat proinsulin-1 gene cDNA engineered to harbor furin consensus cleavage sequences was inserted into EBV-based plasmid vectors that contained CAG promoter or multimerized rat insulin promoter (RIP). Normal or streptozotocin (STZ)-induced diabetic mice were given an injection of the plasmids via the tail vein under high pressure. Transfection of the CAG-proinsulin construct markedly improved hyperglycemia of diabetic mice, accompanied by a considerable increase in serum insulin concentrations. Although the RIP-plasmid failed to reduce fasting blood glucose, the glucose tolerance test and RT-PCR analysis revealed that insulin production was regulated in the liver in a blood glucose level-dependent manner. The present results suggest a potential therapeutic means of controlling DM.Entities:
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Year: 2003 PMID： 14550289 DOI： 10.1016/j.bbrc.2003.09.103
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575