BACKGROUND: High-fat enteral nutrition reduces the inflammatory response following hemorrhagic shock in the rat. AIMS: We hypothesized that this intervention might also ameliorate the remote organ injury to the liver associated with this model. METHODS: Male Sprague-Dawley rats were either starved or fed low-fat or high-fat isocaloric isonitrogenous feed prior to nonlethal hemorrhagic shock induced by a 40% reduction in the blood volume. Animals were sacrificed at 90 minutes or 24 hours after injury. Liver cell damage was assessed by histology and long polymerase chain reaction (PCR) to detect mitochondrial DNA damage. Stress protein expression was measured by Western blot and mRNA expression by real-time PCR and immunohistochemistry. RESULTS: Animals fed a low-fat diet had the same severity of liver injury as starved animals and increased expression of stress proteins. Animals fed a high-fat diet had minimal liver injury, no evidence of mitochondrial DNA damage, and significantly lower expression of stress proteins. This effect is associated with preservation of hepatocellular morphology, attenuation of mitochondrial DNA damage, and a reduced stress protein response to injury. CONCLUSIONS: High-fat enteral nutrition protects the liver from the remote effects of hemorrhagic shock, but the mechanism of this effect is not yet known.
BACKGROUND: High-fat enteral nutrition reduces the inflammatory response following hemorrhagic shock in the rat. AIMS: We hypothesized that this intervention might also ameliorate the remote organ injury to the liver associated with this model. METHODS: Male Sprague-Dawley rats were either starved or fed low-fat or high-fat isocaloric isonitrogenous feed prior to nonlethal hemorrhagic shock induced by a 40% reduction in the blood volume. Animals were sacrificed at 90 minutes or 24 hours after injury. Liver cell damage was assessed by histology and long polymerase chain reaction (PCR) to detect mitochondrial DNA damage. Stress protein expression was measured by Western blot and mRNA expression by real-time PCR and immunohistochemistry. RESULTS: Animals fed a low-fat diet had the same severity of liver injury as starved animals and increased expression of stress proteins. Animals fed a high-fat diet had minimal liver injury, no evidence of mitochondrial DNA damage, and significantly lower expression of stress proteins. This effect is associated with preservation of hepatocellular morphology, attenuation of mitochondrial DNA damage, and a reduced stress protein response to injury. CONCLUSIONS: High-fat enteral nutrition protects the liver from the remote effects of hemorrhagic shock, but the mechanism of this effect is not yet known.
Authors: Y Mizushima; P Wang; D Jarrar; W G Cioffi; K I Bland; I H Chaudry Journal: Am J Physiol Regul Integr Comp Physiol Date: 2000-02 Impact factor: 3.619
Authors: Anita C E Vreugdenhil; Corine H Rousseau; Thomas Hartung; Jan Willem M Greve; Cornelis van 't Veer; Wim A Buurman Journal: J Immunol Date: 2003-02-01 Impact factor: 5.422
Authors: Leo E Otterbein; Brian S Zuckerbraun; Manabu Haga; Fang Liu; Ruiping Song; Anny Usheva; Christina Stachulak; Natalya Bodyak; R Neal Smith; Eva Csizmadia; Shivraj Tyagi; Yorihiro Akamatsu; Richard J Flavell; Timothy R Billiar; Edith Tzeng; Fritz H Bach; Augustine M K Choi; Miguel P Soares Journal: Nat Med Date: 2003-01-21 Impact factor: 53.440
Authors: Misha D P Luyer; Wim A Buurman; M'hamed Hadfoune; Jan A Jacobs; Sergey R Konstantinov; Cornelis H C Dejong; Jan Willem M Greve Journal: Shock Date: 2004-01 Impact factor: 3.454
Authors: Misha D Luyer; Jan Willem M Greve; M'hamed Hadfoune; Jan A Jacobs; Cornelis H Dejong; Wim A Buurman Journal: J Exp Med Date: 2005-10-10 Impact factor: 14.307