Utilizing unsymmetrical indirect covariance processing to define 15N- 13C connectivity networks.

There has been considerable interest over the past decade in the utilization of direct and long-range 1H- 15N heteronuclear shift correlation methods at natural abundance to facilitate the elucidation of small molecule structures. Recently, there has also been a high level of interest in the exploration of indirect covariance NMR methods. Our initial explorations in this area led to the development of unsymmetrical indirect covariance methods, which allow the calculation of hyphenated 2D NMR spectra such as 2D GHSQC-COSY and GHSQC-NOESY from the discrete component 2D NMR experiments. We now wish to report the utilization of unsymmetrical indirect covariance NMR methods for the combination of 1H- 13C GHSQC and 1H- 15N long-range (GHMBC, IMPEACH-MBC, CIGAR-HMBC, etc.) heteronuclear chemical shift correlation spectra to determine 15N- 13C correlation pathways. Copyright 2007 John Wiley & Sons, Ltd.