From lignins to tannins: forty years of enzyme studies on the biosynthesis of phenolic compounds.

In the early 1960s, enzyme studies increasingly began to replace the common 'feeding' experiments in which labeled tracers were applied to living plants or plant parts for elucidating metabolic pathways. This advanced technique allowed to gain much deeper insights into individual details of metabolic sequences, and particularly on the previously inaccessible role of activated 'energy-rich' intermediates. Based on the author's own experience for the past 40+ years in this field, principal findings and trends elucidating the pathways to lignin and lignin precursors, acyl amides and hydrolyzable tannins (gallotannins, ellagitannins) by enzyme studies are reported.