Literature DB >> 17554575

A cold-active esterase of Streptomyces coelicolor A3(2): from genome sequence to enzyme activity.

Sameh H Soror1, V Verma, Ren Rao, Shafaq Rasool, S Koul, G N Qazi, John Cullum.   

Abstract

The genome sequence of Streptomyces coelicolor A3(2) contains 51 putative lipase and esterase genes mostly of unknown function. The gene estB (locus SCO 6966) was expressed as a His-tagged protein in E. coli. Esterase B was active at low temperatures exerting its maximum activity at 30 degrees C and retaining more than 25% of its activity at 4 degrees C. The optimum pH was 8-8.5. The enzyme was active against short synthetic p-nitrophenylesters (C2-C10) with maximum activity towards the acetate ester (C2). The esterase was tested on 13 series of racemic esters of potential interest for the synthesis of chiral pharmaceutical compounds. 4 of the series were substrates and a modest degree of enantioselectivity was observed (enantiomeric ratios of 1.1-1.9).

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Year:  2007        PMID： 17554575     DOI： 10.1007/s10295-007-0224-6

Source DB:  PubMed          Journal:  J Ind Microbiol Biotechnol        ISSN： 1367-5435            Impact factor:   4.258


  36 in total

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Authors:  J P HORWITZ; J CHUA; R J CURBY; A J TOMSON; M A DAROOGE; B E FISHER; J MAURICIO; I KLUNDT
Journal:  J Med Chem       Date:  1964-07       Impact factor: 7.446

2.  Cloning, sequencing, and regulation of expression of an extracellular esterase gene from the plant pathogen Streptomyces scabies.

Authors:  G Raymer; J M Willard; J L Schottel
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

Review 3.  Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases.

Authors:  K E Jaeger; B W Dijkstra; M T Reetz
Journal:  Annu Rev Microbiol       Date:  1999       Impact factor: 15.500

4.  Improved tools for biological sequence comparison.

Authors:  W R Pearson; D J Lipman
Journal:  Proc Natl Acad Sci U S A       Date:  1988-04       Impact factor: 11.205

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Affinity purification and characterization of a cutinase from the fungal plant pathogen Monilinia fructicola (Wint.) honey.

Authors:  G Y Wang; T J Michailides; B D Hammock; Y M Lee; R M Bostock
Journal:  Arch Biochem Biophys       Date:  2000-10-01       Impact factor: 4.013

7.  Synthesis and metabolism of naphthyl substituted phosphoramidate derivatives of stavudine.

Authors:  T K Venkatachalam; S Qazi; F M Uckun
Journal:  Bioorg Med Chem       Date:  2006-05-11       Impact factor: 3.641

8.  The Streptomyces coelicolor A3(2) lipAR operon encodes an extracellular lipase and a new type of transcriptional regulator.

Authors:  Fabiola Valdez; Gabriela González-Cerón; Helen M Kieser; Luis Servı N-González
Journal:  Microbiology       Date:  1999-09       Impact factor: 2.777

9.  A novel streptomycete lipase: cloning, sequencing and high-level expression of the Streptomyces rimosus GDS(L)-lipase gene.

Authors:  Dusica Vujaklija; Werner Schröder; Marija Abramić; Peijian Zou; Ivana Lescić; Peter Franke; Jasenka Pigac
Journal:  Arch Microbiol       Date:  2002-05-16       Impact factor: 2.552

10.  Biochemical and molecular characterization of the extracellular esterase from Streptomyces diastatochromogenes.

Authors:  C Tesch; K Nikoleit; V Gnau; F Götz; C Bormann
Journal:  J Bacteriol       Date:  1996-04       Impact factor: 3.490
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  6 in total

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Authors:  Xin Chang; Shuang Wu; Jie Chen; Shengqi Xiong; Peng Wang; Xueqin Shi; Ao Wang; Baojuan Wang
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2.  Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A covalently inhibited by monoethylphosphonate.

Authors:  Anna Brzuszkiewicz; Elzbieta Nowak; Zbigniew Dauter; Mirosława Dauter; Hubert Cieśliński; Anna Długołecka; Józef Kur
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-08-20

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Journal:  Microb Cell Fact       Date:  2011-05-29       Impact factor: 5.328

4.  Isolation and characterization of EstC, a new cold-active esterase from Streptomyces coelicolor A3(2).

Authors:  Guillaume Brault; François Shareck; Yves Hurtubise; François Lépine; Nicolas Doucet
Journal:  PLoS One       Date:  2012-03-02       Impact factor: 3.240

5.  Characterization of a Novel Moderately Thermophilic Solvent-Tolerant Esterase Isolated From a Compost Metagenome Library.

Authors:  Ji-Min Park; Chul-Hyung Kang; Sung-Min Won; Ki-Hoon Oh; Jung-Hoon Yoon
Journal:  Front Microbiol       Date:  2020-01-24       Impact factor: 5.640

6.  A genomic search approach to identify esterases in Propionibacterium freudenreichii involved in the formation of flavour in Emmental cheese.

Authors:  Julien Dherbécourt; Hélène Falentin; Stéphane Canaan; Anne Thierry
Journal:  Microb Cell Fact       Date:  2008-05-22       Impact factor: 5.328
  6 in total

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