Literature DB >> 17545973

Isolation of renal proximal tubular brush-border membranes.

Jürg Biber1, Bruno Stieger, Gerti Stange, Heini Murer.   

Abstract

This protocol describes a method for the isolation and purification of renal proximal tubular brush-border membranes in high yield and high purity. Based on a different reactivity of the brush-border membrane compared to other cellular membranes with divalent cations, such as Mg2+, purified membrane vesicles can be obtained after a few differential centrifugation steps (within approximately 3 h) that are suitable for in vitro studies, such as transport experiments or protein and lipid analysis.

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Year:  2007        PMID： 17545973     DOI： 10.1038/nprot.2007.156

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  51 in total

1.  Impaired nutrient signaling and body weight control in a Na+ neutral amino acid cotransporter (Slc6a19)-deficient mouse.

Authors:  Angelika Bröer; Torsten Juelich; Jessica M Vanslambrouck; Nadine Tietze; Peter S Solomon; Jeff Holst; Charles G Bailey; John E J Rasko; Stefan Bröer
Journal:  J Biol Chem       Date:  2011-06-02       Impact factor: 5.157

2.  PKB/SGK-resistant GSK3 enhances phosphaturia and calciuria.

Authors:  Michael Föller; Daniela S Kempe; Krishna M Boini; Ganesh Pathare; Balasaheb Siraskar; Paola Capuano; Ioana Alesutan; Mentor Sopjani; Gerti Stange; Nilufar Mohebbi; Madhuri Bhandaru; Teresa F Ackermann; Martin S Judenhofer; Bernd J Pichler; Jürg Biber; Carsten A Wagner; Florian Lang
Journal:  J Am Soc Nephrol       Date:  2011-04-14       Impact factor: 10.121

3.  Expression of renal and intestinal Na/Pi cotransporters in the absence of GABARAP.

Authors:  Sonja C Reining; Annette Liesegang; Heinrich Betz; Jürg Biber; Heini Murer; Nati Hernando
Journal:  Pflugers Arch       Date:  2010-03-31       Impact factor: 3.657

4.  Proteomic analysis of brush-border membrane vesicles isolated from purified proximal convoluted tubules.

Authors:  Scott J Walmsley; Corey Broeckling; Ann Hess; Jessica Prenni; Norman P Curthoys
Journal:  Am J Physiol Renal Physiol       Date:  2010-03-10

5.  Acute parathyroid hormone differentially regulates renal brush border membrane phosphate cotransporters.

Authors:  Nicolas Picard; Paola Capuano; Gerti Stange; Marija Mihailova; Brigitte Kaissling; Heini Murer; Jürg Biber; Carsten A Wagner
Journal:  Pflugers Arch       Date:  2010-06-05       Impact factor: 3.657

6.  A G protein-coupled, IP3/protein kinase C pathway controlling the synthesis of phosphaturic hormone FGF23.

Authors:  Qing He; Lauren T Shumate; Julia Matthias; Cumhur Aydin; Marc N Wein; Jordan M Spatz; Regina Goetz; Moosa Mohammadi; Antonius Plagge; Paola Divieti Pajevic; Murat Bastepe
Journal:  JCI Insight       Date:  2019-09-05

7.  Checkpoint kinase Chk2 controls renal Cyp27b1 expression, calcitriol formation, and calcium-phosphate metabolism.

Authors:  Hajar Fahkri; Bingbing Zhang; Abul Fajol; Nati Hernando; Bernat Elvira; Julia G Mannheim; Bernd J Pichler; Christoph Daniel; Kerstin Amann; Atsushi Hirao; Jillian Haight; Tak W Mak; Florian Lang; Michael Föller
Journal:  Pflugers Arch       Date:  2014-10-17       Impact factor: 3.657

8.  Fluorescent ligand-directed co-localization of the parathyroid hormone 1 receptor with the brush-border scaffold complex of the proximal tubule reveals hormone-dependent changes in ezrin immunoreactivity consistent with inactivation.

Authors:  Jun Guo; Lige Song; Minlin Liu; Matthew J Mahon
Journal:  Biochim Biophys Acta       Date:  2012-10-02

9.  The Na+-Pi cotransporter PiT-2 (SLC20A2) is expressed in the apical membrane of rat renal proximal tubules and regulated by dietary Pi.

Authors:  Ricardo Villa-Bellosta; Silvia Ravera; Victor Sorribas; Gerti Stange; Moshe Levi; Heini Murer; Jürg Biber; Ian C Forster
Journal:  Am J Physiol Renal Physiol       Date:  2008-12-10

10.  Differential regulation of the renal sodium-phosphate cotransporters NaPi-IIa, NaPi-IIc, and PiT-2 in dietary potassium deficiency.

Authors:  Sophia Y Breusegem; Hideaki Takahashi; Hector Giral-Arnal; Xiaoxin Wang; Tao Jiang; Jill W Verlander; Paul Wilson; Shinobu Miyazaki-Anzai; Eileen Sutherland; Yupanqui Caldas; Judith T Blaine; Hiroko Segawa; Ken-ichi Miyamoto; Nicholas P Barry; Moshe Levi
Journal:  Am J Physiol Renal Physiol       Date:  2009-06-03
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