PURPOSE: To test, in vitro, the null hypothesis that there was no difference in the ultrastructure of adhesive-bonded, acid-etched dentin aged under accelerated conditions in mineral oil or artificial saliva. METHODS: Beams of human dentin bonded with three total-etch adhesives were retrieved from the two storage media after 3 years and prepared for transmission electron microscopy. RESULTS: Hybrid layers from specimens aged in mineral oil exhibited structural integrity of the collagen network. Conversely, abnormal hybrid layers were seen in specimens aged in artificial saliva, with progressive disintegration of the fibrillar network to the extent that it was beyond detection by collagen staining. Self-destruction of collagen matrices can occur in resin-infiltrated dentin, and to a lesser degree, in mineralized dentin in the absence of bacterial or salivary enzymes.
RCT Entities:
PURPOSE: To test, in vitro, the null hypothesis that there was no difference in the ultrastructure of adhesive-bonded, acid-etched dentin aged under accelerated conditions in mineral oil or artificial saliva. METHODS: Beams of human dentin bonded with three total-etch adhesives were retrieved from the two storage media after 3 years and prepared for transmission electron microscopy. RESULTS: Hybrid layers from specimens aged in mineral oil exhibited structural integrity of the collagen network. Conversely, abnormal hybrid layers were seen in specimens aged in artificial saliva, with progressive disintegration of the fibrillar network to the extent that it was beyond detection by collagen staining. Self-destruction of collagen matrices can occur in resin-infiltrated dentin, and to a lesser degree, in mineralized dentin in the absence of bacterial or salivary enzymes.
Authors: A Mazzoni; F D Nascimento; M Carrilho; I Tersariol; V Papa; L Tjäderhane; R Di Lenarda; F R Tay; D H Pashley; L Breschi Journal: J Dent Res Date: 2012-02-21 Impact factor: 6.116
Authors: K Hosaka; Y Nishitani; J Tagami; M Yoshiyama; W W Brackett; K A Agee; F R Tay; D H Pashley Journal: J Dent Res Date: 2009-02 Impact factor: 6.116
Authors: Leo Tjäderhane; Fabio D Nascimento; Lorenzo Breschi; Annalisa Mazzoni; Ivarne L S Tersariol; Saulo Geraldeli; Arzu Tezvergil-Mutluay; Marcela Carrilho; Ricardo M Carvalho; Franklin R Tay; David H Pashley Journal: Dent Mater Date: 2013-08-14 Impact factor: 5.304
Authors: M R O Carrilho; R M Carvalho; M F de Goes; V di Hipólito; S Geraldeli; F R Tay; D H Pashley; L Tjäderhane Journal: J Dent Res Date: 2007-01 Impact factor: 6.116
Authors: Arzu Tezvergil-Mutluay; M Murat Mutluay; Li-Sha Gu; Kai Zhang; Kelli A Agee; Ricardo M Carvalho; Adriana Manso; Marcela Carrilho; Franklin R Tay; Lorenzo Breschi; Byoung-In Suh; David H Pashley Journal: J Dent Date: 2010-10-14 Impact factor: 4.379
Authors: Marcela R Carrilho; Franklin R Tay; Adam M Donnelly; Kelli A Agee; Leo Tjäderhane; Annalisa Mazzoni; Lorenzo Breschi; Stephen Foulger; David H Pashley Journal: J Biomed Mater Res B Appl Biomater Date: 2009-07 Impact factor: 3.368
Authors: Lorenzo Breschi; Annalisa Mazzoni; Fernando Nato; Marcela Carrilho; Erika Visintini; Leo Tjäderhane; Alessandra Ruggeri; Franklin R Tay; Elettra De Stefano Dorigo; David H Pashley Journal: Dent Mater Date: 2009-12-31 Impact factor: 5.304