| Literature DB >> 17537596 |
Ajay Pal Singh Aswal1, Sarvesh Raghav, Sachinandan De, Manish Thakur, Surender Lal Goswami, Tirtha Kumar Datta.
Abstract
The present study was undertaken to evaluate the expression stability of two housekeeping genes (HKGs), 18S rRNA and G3PDH during in vitro maturation (IVM) of oocytes in buffalo, which qualifies their use as internal controls for valid qRT-PCR estimation of other oocyte transcripts. A semi quantitative RT-PCR system was used with optimised qRT-PCR parameters at exponential PCR cycle for evaluation of temporal expression pattern of these genes over 24 h of IVM. 18S rRNA was found more stable in its expression pattern than G3PDH.Entities:
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Year: 2007 PMID： 17537596 DOI： 10.1016/j.anireprosci.2007.04.012
Source DB: PubMed Journal: Anim Reprod Sci ISSN： 0378-4320 Impact factor: 2.145