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Literature DB >> 175259

DNA-cellulose column chromatography of phosphorylated nucleolar nonhistone proteins.

Abstract

A salt-extraction procedure was used to isolate a nucleolar nonhistone protein fraction, containing [32P]phosphoserine, from the nucleoli of Novikoff hepatoma ascites cells. These proteins are similar in amino-acid composition to whole nuclear (chromosomal) nonhistone proteins. DNA-cellulose column chromatography showed that this fraction contains DNA-binding phosphoproteins, some of which will bind only to homologous (Novikoff) nucleolar or nuclear DNA.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1976 PMID： 175259 DOI： 10.1007/bf01731675

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

33 in total

1. REGULATION OF RNA SYNTHESIS IN ISOLATED NUCLEOLI BY HISTONES AND NUCLEOLAR PROTEINS.

Authors: M C LIAU; L S HNILICA; R B HURLBERT
Journal: Proc Natl Acad Sci U S A Date: 1965-03 Impact factor: 11.205

2. Serine phosphoric acid from diisopropylphosphoryl chymotrypsin.

Authors: N K SCHAFFER; S C MAY; W H SUMMERSON
Journal: J Biol Chem Date: 1953-05 Impact factor: 5.157

3. Specific binding of phosphorylated non-histone chromatin proteins to deoxyribonucleic acid.

Authors: L J Kleinsmith
Journal: J Biol Chem Date: 1973-08-25 Impact factor: 5.157

4. Similarity of ribosomal and ribosomal precursor RNA's from rat liver and the Novikoff ascites hepatoma.

Authors: T O Sitz; R N Nazar; W H Spohn; H Busch
Journal: Cancer Res Date: 1973-12 Impact factor: 12.701

5. Role of cyclic adenosine 3',5'-monophosphate in the induction of hepatic enzymes. II. Effect of N6,O2'-dibutyryl cyclic adenosine 3'-5'-monophosphate on the kinetics of ribonucleic acid synthesis in purified rat liver nuclei.

Authors: J P Jost; M K Sahib
Journal: J Biol Chem Date: 1971-03-25 Impact factor: 5.157

6. Studies of nuclear acidic proteins. Evidence for their phosphorylation, tissue specificity, selective binding to deoxyribonucleic acid, and stimulation effects on transcription.

Authors: C S Teng; C T Teng; V G Allfrey
Journal: J Biol Chem Date: 1971-06-10 Impact factor: 5.157

DNA-cellulose column chromatography of phosphorylated nucleolar nonhistone proteins.

1. REGULATION OF RNA SYNTHESIS IN ISOLATED NUCLEOLI BY HISTONES AND NUCLEOLAR PROTEINS.

2. Serine phosphoric acid from diisopropylphosphoryl chymotrypsin.

3. Specific binding of phosphorylated non-histone chromatin proteins to deoxyribonucleic acid.

4. Similarity of ribosomal and ribosomal precursor RNA's from rat liver and the Novikoff ascites hepatoma.

5. Role of cyclic adenosine 3',5'-monophosphate in the induction of hepatic enzymes. II. Effect of N6,O2'-dibutyryl cyclic adenosine 3'-5'-monophosphate on the kinetics of ribonucleic acid synthesis in purified rat liver nuclei.

6. Studies of nuclear acidic proteins. Evidence for their phosphorylation, tissue specificity, selective binding to deoxyribonucleic acid, and stimulation effects on transcription.

7. Cytoplasmic DNA-binding proteins.

8. Pre-ribosomal RNA synthesis in isolated rat-liver nucleoli.

9. On the structural organization of operon and the regulation of RNA synthesis in animal cells.

10. Cortisone stimulation of nucleolar RNA polymerase activity.

1. Affinity chromatography of nonhistone chromosomal proteins from lymphocytes on DNA-agarose columns.