Literature DB >> 565462

Affinity chromatography of nonhistone chromosomal proteins from lymphocytes on DNA-agarose columns.

H Blüthmann.   

Abstract

A two step procedure is presented consisting of hydroxyapatite and DNA-agarose chromatography which allows the isolation of nonhistone chromosomal proteins with different affinities towards single stranded DNA. The application of this fractionation scheme to nonhistone chromosomal proteins from bovine lymphocytes is described.

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Year:  1978        PMID: 565462     DOI: 10.1007/bf00775178

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN: 0301-4851            Impact factor:   2.316


  11 in total

1.  Some properties of a DNA-unwinding protein unique to lymphocytes from chronic lymphocytic leukemia.

Authors:  A T Huang; M M Riddle; L S Koons
Journal:  Cancer Res       Date:  1975-04       Impact factor: 12.701

2.  Non-histone chromosomal proteins. Their isolation and role in determining specificity of transcription in vitro.

Authors:  H Blüthmann; S Mrozek; A Gierer
Journal:  Eur J Biochem       Date:  1975-10-15

3.  Specific binding of phosphorylated non-histone chromatin proteins to deoxyribonucleic acid.

Authors:  L J Kleinsmith
Journal:  J Biol Chem       Date:  1973-08-25       Impact factor: 5.157

4.  A rapid, sensitive, and specific method for the determination of protein in dilute solution.

Authors:  W Schaffner; C Weissmann
Journal:  Anal Biochem       Date:  1973-12       Impact factor: 3.365

5.  Phosphorylation of DNA-binding nuclear acidic proteins and gene activation in the HeLa cell cycle.

Authors:  V G Allfrey; A Inoue; J Karn; E M Johnson; G Vidali
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974

6.  Isolation, purification, and fractionation of nonhistone chromosomal proteins.

Authors:  H W van den Broek; L D Noodén; J S Sevall; J Bonner
Journal:  Biochemistry       Date:  1973-01-16       Impact factor: 3.162

7.  Affinity chromatography of DNA-binding enzymes on single-stranded DNA-agarose columns.

Authors:  H Schaller; C Nüsslein; F J Bonhoeffer; C Kurz; I Nietzschmann
Journal:  Eur J Biochem       Date:  1972-04-24

8.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

9.  Specific binding of a nonhistone chromosomal protein from lymphocyte to DNA.

Authors:  H Blüthmann
Journal:  Eur J Biochem       Date:  1976-11-01

10.  Purification and physical characterization of nucleic acid helix-unwinding proteins from calf thymus.

Authors:  G Herrick; B Alberts
Journal:  J Biol Chem       Date:  1976-04-10       Impact factor: 5.157

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  1 in total

1.  DNA-binding properties of a nonhistone chromosomal protein from lymphocytes.

Authors:  H Blüthmann
Journal:  Mol Cell Biochem       Date:  1978-05-31       Impact factor: 3.396

  1 in total

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