BACKGROUND: Methods for the measurement of chorionic gonadotropin for pregnancy detection in laboratory macaques chorionic gonadotropin (mCG) have been limited by the paucity of specific reagents. Current assays use reagents developed for human chorionic gonadotropin (hCG) assays and have been limited to radio- or enzyme-immuno assays. METHODS: This report describes an automated assay for mCG using reagents for hCG detection that has been adapted to the automated chemiluminescence platform of the Bayer ACS-180 autoanalyzer. RESULTS: This automated assay performs similarly to previous assays in terms of sensitivity and specificity but provides additional speed, reliability, and more easily accessible reagents compared with previous formats. CONCLUSIONS: When implemented broadly, this assay could provide standardization of mCG measurements for both research and colony management purposes.
BACKGROUND: Methods for the measurement of chorionic gonadotropin for pregnancy detection in laboratory macaques chorionic gonadotropin (mCG) have been limited by the paucity of specific reagents. Current assays use reagents developed for human chorionic gonadotropin (hCG) assays and have been limited to radio- or enzyme-immuno assays. METHODS: This report describes an automated assay for mCG using reagents for hCG detection that has been adapted to the automated chemiluminescence platform of the Bayer ACS-180 autoanalyzer. RESULTS: This automated assay performs similarly to previous assays in terms of sensitivity and specificity but provides additional speed, reliability, and more easily accessible reagents compared with previous formats. CONCLUSIONS: When implemented broadly, this assay could provide standardization of mCG measurements for both research and colony management purposes.
Authors: Bryn E Willson; Nancy A Gee; Neil H Willits; Lijuan Li; Qi Zhang; Kent E Pinkerton; Bill L Lasley Journal: Reprod Toxicol Date: 2021-08-25 Impact factor: 3.143