PURPOSE: We tested our hypothesis that testosterone increases the meibomian gland gene expression of numerous enzymes in the cholesterol biosynthetic pathway. METHODS: Meibomian glands were obtained from castrated mice treated with vehicle or testosterone for 2 weeks. Tissues were processed for the analysis of selected mRNAs by real-time PCR. RESULTS: Our research demonstrates that testosterone stimulates a significant increase in the mRNA levels of mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylase, isopentenyl pyrophosphate isomerase, geranylgeranyl pyrophosphate synthase, squalene epoxidase, lanosterol synthase, lanosterol demethylase, and Delta 7-sterol reductase. CONCLUSIONS: Our findings indicate that androgens may promote cholesterol biosynthesis in the meibomian gland.
PURPOSE: We tested our hypothesis that testosterone increases the meibomian gland gene expression of numerous enzymes in the cholesterol biosynthetic pathway. METHODS: Meibomian glands were obtained from castrated mice treated with vehicle or testosterone for 2 weeks. Tissues were processed for the analysis of selected mRNAs by real-time PCR. RESULTS: Our research demonstrates that testosterone stimulates a significant increase in the mRNA levels of mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylase, isopentenyl pyrophosphate isomerase, geranylgeranyl pyrophosphate synthase, squalene epoxidase, lanosterol synthase, lanosterol demethylase, and Delta 7-sterol reductase. CONCLUSIONS: Our findings indicate that androgens may promote cholesterol biosynthesis in the meibomian gland.
Authors: Erich Knop; Nadja Knop; Thomas Millar; Hiroto Obata; David A Sullivan Journal: Invest Ophthalmol Vis Sci Date: 2011-03-30 Impact factor: 4.799
Authors: Xiaomin Chen; Benjamin D Sullivan; Raheleh Rahimi Darabad; Shaohui Liu; Wendy R Kam; David A Sullivan Journal: Cornea Date: 2019-12 Impact factor: 2.651
Authors: David A Sullivan; Yang Liu; Wendy R Kam; Juan Ding; Karin M Green; Scott A Shaffer; Mark P Hatton; Shaohui Liu Journal: Invest Ophthalmol Vis Sci Date: 2014-05-27 Impact factor: 4.799