| Literature DB >> 17512533 |
Joan A Aebig1, Gregory E D Mullen, Gelu Dobrescu, Kelly Rausch, Lynn Lambert, Olubunmi Ajose-Popoola, Carole A Long, Allan Saul, Aaron P Miles.
Abstract
CpG oligodeoxynucleotides are potent immunostimulants. For parenterally delivered alum-based vaccines, the immunostimulatory effect of CpG depends on the association of the CpG and antigen to the alum. We describe effects of buffer components on the binding of CPG 7909 to aluminum hydroxide (Alhydrogel), assays for measuring binding of CPG 7909 to alum and CPG 7909 induced dissociation of antigen from the alum. Free CPG 7909 is a potent inducer of IP-10 in mice. However the lack of IP-10 production from formulations containing bound CPG 7909 suggested that CPG 7909 does not rapidly dissociate from the alum after injection. It also suggests that IP-10 assays are not a good basis for potency assays for alum-based vaccines containing CPG 7909.Entities:
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Year: 2007 PMID： 17512533 PMCID： PMC2001166 DOI： 10.1016/j.jim.2007.04.003
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303