| Literature DB >> 19146977 |
Feng Qian1, Joan A Aebig, Karine Reiter, Emma Barnafo, Yanling Zhang, Richard L Shimp, Kelly M Rausch, David S Jones, Daming Zhu, Lynn Lambert, Gregory E D Mullen, David L Narum, Louis H Miller, Yimin Wu.
Abstract
In this paper we report our efforts to enhance the immunogenicity of Pfs28, a transmission blocking vaccine candidate of Plasmodium falciparum, using a strategy of chemical conjugation. With an improved procedure, Pfs28 was covalently coupled to the mutant and non-toxic ExoProtein A of Pseudomonas aeruginosa by the reaction between thiolated antigen and maleimide modified carrier protein. The optimized process resulted in a higher antigen-carrier conjugation ratio, and the conjugation product could be purified using single-step size-exclusion chromatography. A significant increase in immunogenicity measured by ELISA was observed in mice immunized with conjugated Pfs28 as compared to unconjugated Pfs28.Entities:
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Year: 2008 PMID： 19146977 PMCID： PMC2761608 DOI： 10.1016/j.micinf.2008.12.009
Source DB: PubMed Journal: Microbes Infect ISSN： 1286-4579 Impact factor: 2.700