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Literature DB >> 1750671

Detection of poly(ADP ribose) polymerase in crude extracts by activity-blot.

F Simonin¹, J P Briand, S Muller, G de Murcia.

Abstract

We have recently devised an activity-blot procedure permitting the detection, on the same nitrocellulose sheet, of the functional poly(ADP ribose) polymerase (PARP) activity as well as the immunostained active peptide(s) after renaturation of the transferred protein(s). Using this technique we have analyzed the PARP activity in higher and lower eukaryotes directly on crude extracts from cell cultures. This procedure has been extended also to in situ screening of bacterial colonies expressing the PARP enzymatic activity.

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Year: 1991 PMID： 1750671 DOI： 10.1016/0003-2697(91)90321-j

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

15 in total

1. Requirement of poly(ADP-ribose) polymerase in recovery from DNA damage in mice and in cells.

Authors: J M de Murcia; C Niedergang; C Trucco; M Ricoul; B Dutrillaux; M Mark; F J Oliver; M Masson; A Dierich; M LeMeur; C Walztinger; P Chambon; G de Murcia
Journal: Proc Natl Acad Sci U S A Date: 1997-07-08 Impact factor: 11.205

2. XRCC1 is specifically associated with poly(ADP-ribose) polymerase and negatively regulates its activity following DNA damage.

Authors: M Masson; C Niedergang; V Schreiber; S Muller; J Menissier-de Murcia; G de Murcia
Journal: Mol Cell Biol Date: 1998-06 Impact factor: 4.272

3. A dominant-negative mutant of human poly(ADP-ribose) polymerase affects cell recovery, apoptosis, and sister chromatid exchange following DNA damage.

Authors: V Schreiber; D Hunting; C Trucco; B Gowans; D Grunwald; G De Murcia; J M De Murcia
Journal: Proc Natl Acad Sci U S A Date: 1995-05-23 Impact factor: 11.205

4. trans-dominant inhibition of poly(ADP-ribosyl)ation sensitizes cells against gamma-irradiation and N-methyl-N'-nitro-N-nitrosoguanidine but does not limit DNA replication of a polyomavirus replicon.

Authors: J H Küpper; M Müller; M K Jacobson; J Tatsumi-Miyajima; D L Coyle; E L Jacobson; A Bürkle
Journal: Mol Cell Biol Date: 1995-06 Impact factor: 4.272

5. A dual approach in the study of poly (ADP-ribose) polymerase: in vitro random mutagenesis and generation of deficient mice.

Authors: C Trucco; V Rolli; F J Oliver; E Flatter; M Masson; F Dantzer; C Niedergang; B Dutrillaux; J Ménissier-de Murcia; G de Murcia
Journal: Mol Cell Biochem Date: 1999-03 Impact factor: 3.396

Detection of poly(ADP ribose) polymerase in crude extracts by activity-blot.

1. Requirement of poly(ADP-ribose) polymerase in recovery from DNA damage in mice and in cells.

2. XRCC1 is specifically associated with poly(ADP-ribose) polymerase and negatively regulates its activity following DNA damage.

3. A dominant-negative mutant of human poly(ADP-ribose) polymerase affects cell recovery, apoptosis, and sister chromatid exchange following DNA damage.

4. trans-dominant inhibition of poly(ADP-ribosyl)ation sensitizes cells against gamma-irradiation and N-methyl-N'-nitro-N-nitrosoguanidine but does not limit DNA replication of a polyomavirus replicon.

5. A dual approach in the study of poly (ADP-ribose) polymerase: in vitro random mutagenesis and generation of deficient mice.

6. Detection of poly(ADP-ribose) polymerase and its reaction product poly(ADP-ribose) by immunocytochemistry.

7. Purification and characterization of NAD+:ADP-ribosyltransferase (polymerizing) from Dictyostelium discoideum.

8. Nicotinamide inhibits nitric oxide synthase mRNA induction in activated macrophages.

Review 9. Structure and function of poly(ADP-ribose) polymerase.

10. Purification and cDNA cloning of maize Poly(ADP)-ribose polymerase.