Literature DB >> 1750668

Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerases.

V V Gurevich1, I D Pokrovskaya, T A Obukhova, S A Zozulya.   

Abstract

A method for preparative in vitro mRNA synthesis is presented. The method makes it possible to generate several hundred RNA copies per molecule of linearized DNA template. The protocol is applicable to the synthesis of RNAs of differing lengths (from 200 to 3000 bases), and both SP6 and T7 RNA polymerases can be used. The transcription can be scaled up to at least 6-12 ml, yielding 4.5-18 mg of homogeneous biologically active mRNA.

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Year:  1991        PMID： 1750668     DOI： 10.1016/0003-2697(91)90318-n

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  45 in total

1.  Hsp47: a molecular chaperone that interacts with and stabilizes correctly-folded procollagen.

Authors:  M Tasab; M R Batten; N J Bulleid
Journal:  EMBO J       Date:  2000-05-15       Impact factor: 11.598

2.  Ribosome-associated protein that inhibits translation at the aminoacyl-tRNA binding stage.

Authors:  D E Agafonov; V A Kolb; A S Spirin
Journal:  EMBO Rep       Date:  2001-05       Impact factor: 8.807

3.  T7 RNA polymerase as a self-replicating label for antigen quantification.

Authors:  Bakhos A Tannous; Eleftheria Laios; Theodore K Christopoulos
Journal:  Nucleic Acids Res       Date:  2002-12-15       Impact factor: 16.971

4.  Type-III procollagen assembly in semi-intact cells: chain association, nucleation and triple-helix folding do not require formation of inter-chain disulphide bonds but triple-helix nucleation does require hydroxylation.

Authors:  N J Bulleid; R Wilson; J F Lees
Journal:  Biochem J       Date:  1996-07-01       Impact factor: 3.857

5.  Site-directed hydroxyl radical probing of 30S ribosomal subunits by using Fe(II) tethered to an interruption in the 16S rRNA chain.

Authors:  R R Samaha; S Joseph; B O'Brien; T W O'Brien; H F Noller
Journal:  Proc Natl Acad Sci U S A       Date:  1999-01-19       Impact factor: 11.205

6.  Modifications of the 5' cap of mRNAs during Xenopus oocyte maturation: independence from changes in poly(A) length and impact on translation.

Authors:  D L Gillian-Daniel; N K Gray; J Aström; A Barkoff; M Wickens
Journal:  Mol Cell Biol       Date:  1998-10       Impact factor: 4.272

7.  Is protein disulfide isomerase a redox-dependent molecular chaperone?

Authors:  Richard A Lumb; Neil J Bulleid
Journal:  EMBO J       Date:  2002-12-16       Impact factor: 11.598

8.  The ribosome-in-pieces: binding of elongation factor EF-G to oligoribonucleotides that mimic the sarcin/ricin and thiostrepton domains of 23S ribosomal RNA.

Authors:  A Munishkin; I G Wool
Journal:  Proc Natl Acad Sci U S A       Date:  1997-11-11       Impact factor: 11.205

9.  Regulation of the double-stranded RNA-dependent protein kinase PKR by RNAs encoded by a repeated sequence in the Epstein-Barr virus genome.

Authors:  A Elia; K G Laing; A Schofield; V J Tilleray; M J Clemens
Journal:  Nucleic Acids Res       Date:  1996-11-15       Impact factor: 16.971

10.  Two distinct regions in the 3' untranslated region of tumor necrosis factor alpha mRNA form complexes with macrophage proteins.

Authors:  Z Hel; E Skamene; D Radzioch
Journal:  Mol Cell Biol       Date:  1996-10       Impact factor: 4.272
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