OBJECTIVES: To investigate the effectiveness of human amniotic membrane (HAM) in the prevention of postoperative adhesion formation and to compare it with the efficacy of hyaluronate/carboxymethylcellulose (HA/CMC) membrane in a rat model. METHODS: Following pilot studies and computer-generated randomization, 23 female Wistar albino rats were operated on in the full study. One of the uterine horns with standard lesions was treated with either HAM (n = 13) or HA/CMC (n = 10) and the other uterine horn served as the control. Second look laparotomies were performed 2 weeks after the operations. Main outcome measures were extent, severity, degree, total adhesion scores and histopathologic characteristics of adhesions. RESULTS: Uterine horns treated with HAM had significantly lower total adhesion scores than the controls (5.15 +/- 2.67 vs. 7.92 +/- 1.50, P < 0.05). Total adhesion scores of uterine horns treated with HA/CMC membrane were significantly lower than those of the controls (4.30 +/- 1.95 vs. 7.50 +/- 1.84, P < 0.01). There were no significant differences between the HAM and HA/CMC groups regarding any adhesion scores. CONCLUSIONS: HAM and HA/CMC membrane are both effective for prevention of adhesion formation in a rat uterine horn model; however, one does not seem to be more effective than the other.
OBJECTIVES: To investigate the effectiveness of human amniotic membrane (HAM) in the prevention of postoperative adhesion formation and to compare it with the efficacy of hyaluronate/carboxymethylcellulose (HA/CMC) membrane in a rat model. METHODS: Following pilot studies and computer-generated randomization, 23 female Wistar albino rats were operated on in the full study. One of the uterine horns with standard lesions was treated with either HAM (n = 13) or HA/CMC (n = 10) and the other uterine horn served as the control. Second look laparotomies were performed 2 weeks after the operations. Main outcome measures were extent, severity, degree, total adhesion scores and histopathologic characteristics of adhesions. RESULTS: Uterine horns treated with HAM had significantly lower total adhesion scores than the controls (5.15 +/- 2.67 vs. 7.92 +/- 1.50, P < 0.05). Total adhesion scores of uterine horns treated with HA/CMC membrane were significantly lower than those of the controls (4.30 +/- 1.95 vs. 7.50 +/- 1.84, P < 0.01). There were no significant differences between the HAM and HA/CMC groups regarding any adhesion scores. CONCLUSIONS: HAM and HA/CMC membrane are both effective for prevention of adhesion formation in a rat uterine horn model; however, one does not seem to be more effective than the other.
Authors: Agnieszka Klama-Baryła; Ewa Rojczyk; Diana Kitala; Wojciech Łabuś; Wojciech Smętek; Katarzyna Wilemska-Kucharzewska; Marek Kucharzewski Journal: Int Wound J Date: 2020-01-15 Impact factor: 3.315
Authors: Joseph Nassif; Sehrish A Abbasi; Mohamad Karim Kechli; Suzan S Boutary; Labib Ghulmiyyah; Ibrahim Khalifeh; Hussein Abou Ghaddara; Anwar H Nassar Journal: Front Med (Lausanne) Date: 2016-03-29