BACKGROUND: This study was aimed at examining whether or not the addition of amniotic membrane to a sutured colonic anastomosis improves its healing. MATERIAL AND METHODS: Ninety female Sprague Dawley rats were used in the study. Ten served as controls for bursting pressure measurement, while the other 80 animals were divided into four groups: Anastomosis group (NA), high-risk anastomosis group (HRA), anastomosis plus amniotic membrane group (NA-AM), and high-risk anastomosis plus amniotic membrane group (HRA-AM). The last two groups had amniotic membrane covering their anastomoses. Anastomotic evaluation was carried out on the third (NA3, HRA3, NA-AM3, and HRA-AM3, respectively) and seventh (NA7, HRA7, NA-AM7, and HRA-AM7, respectively) postoperative days. The main outcome measures were gross anastomotic healing, adhesion formation, mechanical strength, hydroxyproine content, and parameters of histopathological healing. RESULTS: Anastomotic dehiscence rate was 66.7%, 40%, 20%, and 10% in group HRA7, HRA3, NA7, and NA3, respectively. However, there was no significant difference between groups regarding the dehiscence rate. The adhesion scores were significantly higher in groups NA3 and HRA3 compared with groups NA-AM3 and HRA-AM3, respectively (p < 0.05, p < 0.001). Bursting pressure was significantly higher in groups with amniotic membrane compared without amniotic membrane (p < 0.05, for all comparison). Inflammatory cell infiltration was significantly lower in groups with amniotic membrane compared with groups without amniotic membrane (p < 0.05, for all both comparisons). Neoangiogenesis was significantly higher in the NA-AM3 and HRA-AM3 groups compared with the NA3 (p < 0.01) and HRA3 (p < 0.05) groups, respectively. Fibroblast activity was significantly higher in groups NA-AM3 and NA-AM7 compared with groups NA3 (p < 0.05) and NA7 (p < 0.05), respectively. Collagen deposition and hydroxyproline concentrations were significantly higher in groups with amniotic membrane compared with groups without amniotic membrane (p < 0.05, for all both comparisons). CONCLUSION: The covering of both normal and high-risk colonic anastomoses with amniotic membrane provides a beneficial effect over conventional suturing of healing.
BACKGROUND: This study was aimed at examining whether or not the addition of amniotic membrane to a sutured colonic anastomosis improves its healing. MATERIAL AND METHODS: Ninety female Sprague Dawley rats were used in the study. Ten served as controls for bursting pressure measurement, while the other 80 animals were divided into four groups: Anastomosis group (NA), high-risk anastomosis group (HRA), anastomosis plus amniotic membrane group (NA-AM), and high-risk anastomosis plus amniotic membrane group (HRA-AM). The last two groups had amniotic membrane covering their anastomoses. Anastomotic evaluation was carried out on the third (NA3, HRA3, NA-AM3, and HRA-AM3, respectively) and seventh (NA7, HRA7, NA-AM7, and HRA-AM7, respectively) postoperative days. The main outcome measures were gross anastomotic healing, adhesion formation, mechanical strength, hydroxyproine content, and parameters of histopathological healing. RESULTS: Anastomotic dehiscence rate was 66.7%, 40%, 20%, and 10% in group HRA7, HRA3, NA7, and NA3, respectively. However, there was no significant difference between groups regarding the dehiscence rate. The adhesion scores were significantly higher in groups NA3 and HRA3 compared with groups NA-AM3 and HRA-AM3, respectively (p < 0.05, p < 0.001). Bursting pressure was significantly higher in groups with amniotic membrane compared without amniotic membrane (p < 0.05, for all comparison). Inflammatory cell infiltration was significantly lower in groups with amniotic membrane compared with groups without amniotic membrane (p < 0.05, for all both comparisons). Neoangiogenesis was significantly higher in the NA-AM3 and HRA-AM3 groups compared with the NA3 (p < 0.01) and HRA3 (p < 0.05) groups, respectively. Fibroblast activity was significantly higher in groups NA-AM3 and NA-AM7 compared with groups NA3 (p < 0.05) and NA7 (p < 0.05), respectively. Collagen deposition and hydroxyproline concentrations were significantly higher in groups with amniotic membrane compared with groups without amniotic membrane (p < 0.05, for all both comparisons). CONCLUSION: The covering of both normal and high-risk colonic anastomoses with amniotic membrane provides a beneficial effect over conventional suturing of healing.
Authors: I Kanellos; I Mantzoros; H Demetriades; S Kalfadis; T Kelpis; L Sakkas; D Betsis Journal: Dis Colon Rectum Date: 2004-02-26 Impact factor: 4.585
Authors: Marc H Schreinemacher; Johanne G Bloemen; Stijn J van der Heijden; Marion J Gijbels; Cornelis H Dejong; Nicole D Bouvy Journal: Int J Colorectal Dis Date: 2011-02-23 Impact factor: 2.571
Authors: Juan Manuel Suárez-Grau; Carlos Bernardos García; Carmen Cepeda Franco; Cristina Mendez García; Salud García Ruiz; Fernando Docobo Durantez; Salvador Morales-Conde; Javier Padillo Ruiz Journal: World J Gastrointest Surg Date: 2016-09-27
Authors: Sam Moslemi; Sajjad Ahmadi Joraghi; Reza Roshanravan; Leila Ghahramani; Mohammad Mohammadianpanah; Masood Hosseinzadeh; Abbas Rezaianzadeh; Ahmed Mohammed Ali Hussein; Neda Najibpour; Seyed Vahid Hosseini Journal: Iran J Med Sci Date: 2016-11
Authors: Leila Ghahramani; Ali Bagherpour Jahromi; Mohammad Reza Dehghani; Mohammad Javad Ashraf; Salar Rahimikazerooni; Abbas Rezaianzadeh; Ali Reza Safarpour; Seyed Vahid Hosseini Journal: Adv Biomed Res Date: 2014-04-17