AIM: To find a method for isolation and cultivation of human adipose-derived mesenchymal stem cells(ADSCs), and to investigate its chondrogenic ability. METHODS: Liposuction human adipose tissues were digested with collagenase type II. The obtained cells were primarily cultured in DMEM medium, and then changed into the induced medium. Alcian Blue stain, picrosirius red stain and the specific collagenase type II immunohistochemical staining were used to detect the results. RESULTS: After cultured in chondrogenic medium, the cells differentiated toward cartilage cells and secreted the specific cartilaginous matrices sulfated proteglycan and collagen type II. CONCLUSION: The human ADSCs have the potential ability to differentiate toward cartilage cells in the appropriated medium. The result provide a new method for the further research in cartilaginous tissue engineering.
AIM: To find a method for isolation and cultivation of human adipose-derived mesenchymal stem cells(ADSCs), and to investigate its chondrogenic ability. METHODS: Liposuction human adipose tissues were digested with collagenase type II. The obtained cells were primarily cultured in DMEM medium, and then changed into the induced medium. Alcian Blue stain, picrosirius red stain and the specific collagenase type II immunohistochemical staining were used to detect the results. RESULTS: After cultured in chondrogenic medium, the cells differentiated toward cartilage cells and secreted the specific cartilaginous matrices sulfated proteglycan and collagen type II. CONCLUSION: The human ADSCs have the potential ability to differentiate toward cartilage cells in the appropriated medium. The result provide a new method for the further research in cartilaginous tissue engineering.
Authors: Adil M Allahverdiyev; Melahat Bagirova; Serhat Elcicek; Rabia Cakir Koc; Serap Yesilkir Baydar; Necati Findikli; Olga N Oztel Journal: Am J Trop Med Hyg Date: 2011-09 Impact factor: 2.345