AIM: To study the effect on induction of allograft immune tolerance by IL-10 and methylprednisolone (Medron) modified dendritic cells(DC) of donor in murine allogenetic skin transplantation model. METHODS: The recipient BALB/c mice were divided into 11 groups.Except group A, each group had been injected with corresponding donor DC through vena caudalis, and transplanted full-thickness skin through back to back from donor C57BL/6 three days later. Corresponding doses were: A: injected NS, blank control; B: injected non-modified donor DC; C1: 10 microg/L IL-10 group; C2: 30 microg/L IL-10 group; D1: 10 mg/L Medron group; D2: 20 mg/L Medron group; E1-E4: 10 microg/L IL-10+10 mg/L Medron group, 10 microg/L IL-10+20 mg/L Medron group, 30 microg/L IL-10+10 mg/L Medron group and 30 microg/L IL-10+20 mg/L Medron group. F group was skin transplantation between two BALB/c mice. Skin transplantation was performed and the survival time of skin allografts was observed. RESULTS: Among all the groups, the graft of E3 group(30 microg/L IL-10 mg/L Medron) survived for the longest time. Statistical analysis showed that there was interaction between IL-10 and Medron (P<0.05). CONCLUSION: The pretreatment with donor DC treated by IL-10 and Medron can prolong the survival time of murine skin allografts after transplantation and induce immune tolerance successfully.
AIM: To study the effect on induction of allograft immune tolerance by IL-10 and methylprednisolone (Medron) modified dendritic cells(DC) of donor in murine allogenetic skin transplantation model. METHODS: The recipient BALB/c mice were divided into 11 groups.Except group A, each group had been injected with corresponding donor DC through vena caudalis, and transplanted full-thickness skin through back to back from donor C57BL/6 three days later. Corresponding doses were: A: injected NS, blank control; B: injected non-modified donor DC; C1: 10 microg/L IL-10 group; C2: 30 microg/L IL-10 group; D1: 10 mg/L Medron group; D2: 20 mg/L Medron group; E1-E4: 10 microg/L IL-10+10 mg/L Medron group, 10 microg/L IL-10+20 mg/L Medron group, 30 microg/L IL-10+10 mg/L Medron group and 30 microg/L IL-10+20 mg/L Medron group. F group was skin transplantation between two BALB/c mice. Skin transplantation was performed and the survival time of skin allografts was observed. RESULTS: Among all the groups, the graft of E3 group(30 microg/L IL-10 mg/L Medron) survived for the longest time. Statistical analysis showed that there was interaction between IL-10 and Medron (P<0.05). CONCLUSION: The pretreatment with donor DC treated by IL-10 and Medron can prolong the survival time of murine skin allografts after transplantation and induce immune tolerance successfully.