| Literature DB >> 17487549 |
M L Reyes Escogido1, A De León Rodríguez, A P Barba de la Rosa.
Abstract
A novel expression vector (pLR) driven by hup promoter and Bifidobacterium beta-galactosidase signal peptide was constructed. The pLR vector was used for the expression of the optimized human IL-10 synthetic gene in Escherichia coli and Bifidobacterium longum. In both microorganisms, rhIL-10 was in a soluble form in total extract cells. The recombinant hIL-10 was partially processed in E. coli, whereas in Bifidobacterium all rhIL-10 was found in the mature form.Entities:
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Year: 2007 PMID： 17487549 DOI： 10.1007/s10529-007-9376-8
Source DB: PubMed Journal: Biotechnol Lett ISSN： 0141-5492 Impact factor: 2.461