OBJECTIVE: To examine the effects of age and/or diabetes on oxidative stress and steroidogenesis, and the protective effect of vitamin E in testis tissue. DESIGN: Controlled experimental study. SETTING: Pamukkale University School of Medicine animal facility. ANIMAL(S): Male Wistar rats divided into six groups with six animals in each group: young control; young diabetic; young diabetic with vitamin E treatment; aged control; aged diabetic; and aged diabetic with vitamin E treatment. INTERVENTION(S): Diabetes was induced by a single intraperitoneal injection of 50 mg/kg streptozotocin and was confirmed by testing blood glucose levels 5 to 7 days after injection. Vitamin E was administered orally for 6 weeks. MAIN OUTCOME MEASURE(S): Serum testosterone and tissue malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) levels were measured, and testis tissue was examined histopathologically. RESULT(S): Elevated malondialdehyde and reduced superoxide dismutase, glutathione, and serum testosterone levels were detected only in the young and aged-diabetic groups. Histopathologic change was not detected in the testis tissue in any of the groups. CONCLUSION(S): Age does not alter the effects of diabetes-induced free radical damage in testis tissue; improvement in this damage can be achieved by vitamin E treatment.
OBJECTIVE: To examine the effects of age and/or diabetes on oxidative stress and steroidogenesis, and the protective effect of vitamin E in testis tissue. DESIGN: Controlled experimental study. SETTING: Pamukkale University School of Medicine animal facility. ANIMAL(S): Male Wistar rats divided into six groups with six animals in each group: young control; young diabetic; young diabetic with vitamin E treatment; aged control; aged diabetic; and aged diabetic with vitamin E treatment. INTERVENTION(S): Diabetes was induced by a single intraperitoneal injection of 50 mg/kg streptozotocin and was confirmed by testing blood glucose levels 5 to 7 days after injection. Vitamin E was administered orally for 6 weeks. MAIN OUTCOME MEASURE(S): Serum testosterone and tissue malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) levels were measured, and testis tissue was examined histopathologically. RESULT(S): Elevated malondialdehyde and reduced superoxide dismutase, glutathione, and serum testosterone levels were detected only in the young and aged-diabetic groups. Histopathologic change was not detected in the testis tissue in any of the groups. CONCLUSION(S): Age does not alter the effects of diabetes-induced free radical damage in testis tissue; improvement in this damage can be achieved by vitamin E treatment.
Authors: Rosa María Vigueras-Villaseñor; Idahue Ojeda; Oscar Gutierrez-Pérez; Margarita Chavez-Saldaña; Osvaldo Cuevas; Daniel Santa Maria; Julio César Rojas-Castañeda Journal: Int J Exp Pathol Date: 2011-02-12 Impact factor: 1.925
Authors: T Kurosawa; Y Mifune; A Inui; H Nishimoto; Y Ueda; T Kataoka; K Yamaura; S Mukohara; R Kuroda Journal: Bone Joint Res Date: 2020-05-16 Impact factor: 5.853
Authors: Ayodele Jacob Akinyemi; Isaac A Adedara; Gustavo Roberto Thome; Vera Maria Morsch; Monique Tomazele Rovani; Lady Katerine Serrano Mujica; Thiago Duarte; Marta Duarte; Ganiyu Oboh; Maria Rosa Chitolina Schetinger Journal: Toxicol Rep Date: 2015-10-13