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Literature DB >> 17482260

The effect of covalently immobilized rhIL-1ra-ELP fusion protein on the inflammatory profile of LPS-stimulated human monocytes.

Dong-Hwan Kim¹, Jason T Smith, Ashutosh Chilkoti, William M Reichert.

Abstract

The objective of this research was to investigate whether immobilized anti-inflammatory cytokines will signal changes in the inflammatory profile of cultured monocytes. A fusion protein of recombinant human IL-1 receptor antagonist and elastin-like peptide (IL-1ra-ELP) was expressed in Escherichia coli. THP-1 human monocytes were cultured on either carboxyl-terminated self-assembled monolayers (SAMs), or SAMs with either covalently immobilized or soluble IL-1ra-ELP. LPS-stimulated monocytes exposed to either soluble or immobilized IL-1ra-ELP were prevented from cell differentiation, showed attenuated expression of pro-inflammatory cytokines, and had increased production of anti-inflammatory and pro-wound healing cytokines. These results suggest that immobilized anti-inflammatory cytokines have the potential to be immunomodulatory biomaterials.

Entities: Chemical Gene Species

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Year: 2007 PMID： 17482260 PMCID： PMC2680615 DOI： 10.1016/j.biomaterials.2007.04.010

Source DB: PubMed Journal: Biomaterials ISSN： 0142-9612 Impact factor: 12.479

41 in total

1. Purification of recombinant proteins by fusion with thermally-responsive polypeptides.

Authors: D E Meyer; A Chilkoti
Journal: Nat Biotechnol Date: 1999-11 Impact factor: 54.908

2. Cytokine traps: multi-component, high-affinity blockers of cytokine action.

Authors: Aris N Economides; Laura Rocco Carpenter; John S Rudge; Vivien Wong; Ellen M Koehler-Stec; Christopher Hartnett; Erica A Pyles; Xiaobing Xu; Thomas J Daly; Michael R Young; James P Fandl; Frank Lee; Scott Carver; Jennifer McNay; Kevin Bailey; Swayampakula Ramakanth; Renta Hutabarat; Tammy T Huang; Czeslaw Radziejewski; George D Yancopoulos; Neil Stahl
Journal: Nat Med Date: 2002-12-16 Impact factor: 53.440

The effect of covalently immobilized rhIL-1ra-ELP fusion protein on the inflammatory profile of LPS-stimulated human monocytes.

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