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Literature DB >> 17477704

Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells.

Veronica Gomez-Godinez¹, Nicole M Wakida, Alexander S Dvornikov, Kyoko Yokomori, Michael W Berns.

Abstract

An 800-nm 200-fs laser is used to produce DNA damage in rat kangaroo (PtK1) and human cystic fibrosis pancreatic adenoma carcinoma (CFPAC-1) cells. Immunofluorescence staining for DNA repair factors in irradiated cells displays localization of gammaH2AX, Nbs1, and Rad50 to the site of irradiation 3 to 30 min following laser exposure. It is concluded that the 200-fs near-infrared laser is an excellent source for the production and study of spatially defined regions of DNA damage.

Entities: Disease Gene Species

Mesh：

Substances：
DNA-Binding Proteins
DNA

Year: 2007 PMID： 17477704 DOI： 10.1117/1.2717684

Source DB: PubMed Journal: J Biomed Opt ISSN： 1083-3668 Impact factor: 3.170

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Cited

8 in total

1. Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on factor assemblies at DNA damage sites.

Authors: Gladys Mae Saquilabon Cruz; Xiangduo Kong; Bárbara Alcaraz Silva; Nima Khatibzadeh; Ryan Thai; Michael W Berns; Kyoko Yokomori
Journal: Nucleic Acids Res Date: 2015-09-30 Impact factor: 16.971

2. Targeting telomere-containing chromosome ends with a near-infrared femtosecond laser to study the activation of the DNA damage response and DNA damage repair pathways.

Authors: Bárbara Alcaraz Silva; Jessica R Stambaugh; Michael W Berns
Journal: J Biomed Opt Date: 2013-09 Impact factor: 3.170

Recruitment of DNA damage recognition and repair pathway proteins following near-IR femtosecond laser irradiation of cells.

1. Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on factor assemblies at DNA damage sites.

2. Targeting telomere-containing chromosome ends with a near-infrared femtosecond laser to study the activation of the DNA damage response and DNA damage repair pathways.

3. Spatially sculpted laser scissors for study of DNA damage and repair.

4. Chromosome tips damaged in anaphase inhibit cytokinesis.

Review 5. Radiation microbeams as spatial and temporal probes of subcellular and tissue response.

6. Analysis of DNA double-strand break response and chromatin structure in mitosis using laser microirradiation.

7. DNA damage induced during mitosis undergoes DNA repair synthesis.

8. Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells.