Literature DB >> 17476709

iTRAQ compatibility of peptide immobilized pH gradient isoelectric focusing.

Johan Lengqvist1, Kristina Uhlén, Janne Lehtiö.   

Abstract

Immobilized pH gradient isoelectric focusing (IPG-IEF) has emerged as a highly promising alternative to strong-cation exchange fractionation as the first dimension in shot-gun proteomics. Herein is shown the compatibility of this method with iTRAQ isotope labeling for relative quantitation and validation of sequence matches from database searching.

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Year:  2007        PMID： 17476709     DOI： 10.1002/pmic.200600782

Source DB:  PubMed          Journal:  Proteomics        ISSN： 1615-9853            Impact factor:   3.984


  18 in total

1.  Combining isoelectric point-based fractionation, liquid chromatography and mass spectrometry to improve peptide detection and protein identification.

Authors:  Stephanie M Cologna; William K Russell; Peniel J Lim; Gyula Vigh; David H Russell
Journal:  J Am Soc Mass Spectrom       Date:  2010-04-24       Impact factor: 3.109

2.  Proteomic and transcriptomic analysis of heart failure due to volume overload in a rat aorto-caval fistula model provides support for new potential therapeutic targets - monoamine oxidase A and transglutaminase 2.

Authors:  Jiri Petrak; Jana Pospisilova; Miroslava Sedinova; Petr Jedelsky; Lucie Lorkova; Ondrej Vit; Michal Kolar; Hynek Strnad; Jan Benes; David Sedmera; Ludek Cervenka; Vojtech Melenovsky
Journal:  Proteome Sci       Date:  2011-11-11       Impact factor: 2.480

3.  Isobaric labeling and data normalization without requiring protein quantitation.

Authors:  Phillip D Kim; Bhavinkumar B Patel; Anthony T Yeung
Journal:  J Biomol Tech       Date:  2012-04

4.  HiRIEF LC-MS enables deep proteome coverage and unbiased proteogenomics.

Authors:  Rui M M Branca; Lukas M Orre; Henrik J Johansson; Viktor Granholm; Mikael Huss; Åsa Pérez-Bercoff; Jenny Forshed; Lukas Käll; Janne Lehtiö
Journal:  Nat Methods       Date:  2013-11-17       Impact factor: 28.547

5.  Immunodepletion plasma proteomics by tripleTOF 5600 and Orbitrap elite/LTQ-Orbitrap Velos/Q exactive mass spectrometers.

Authors:  Kelly A Jones; Phillip D Kim; Bhavinkumar B Patel; Steven G Kelsen; Alan Braverman; Derrick J Swinton; Philip R Gafken; Lisa A Jones; William S Lane; John M Neveu; Hon-Chiu E Leung; Scott A Shaffer; John D Leszyk; Bruce A Stanley; Todd E Fox; Anne Stanley; Michael J Hall; Heather Hampel; Christopher D South; Albert de la Chapelle; Randall W Burt; David A Jones; Levy Kopelovich; Anthony T Yeung
Journal:  J Proteome Res       Date:  2013-09-19       Impact factor: 4.466

6.  Tumor proteomics by multivariate analysis on individual pathway data for characterization of vulvar cancer phenotypes.

Authors:  Annsofi Sandberg; Gunnel Lindell; Brita Nordström Källström; Rui Mamede Branca; Kristina Gemzell Danielsson; Mats Dahlberg; Barbro Larson; Jenny Forshed; Janne Lehtiö
Journal:  Mol Cell Proteomics       Date:  2012-04-12       Impact factor: 5.911

7.  Integrating proteomic and transcriptomic high-throughput surveys for search of new biomarkers of colon tumors.

Authors:  Michal Mikula; Tymon Rubel; Jakub Karczmarski; Krzysztof Goryca; Michal Dadlez; Jerzy Ostrowski
Journal:  Funct Integr Genomics       Date:  2010-11-09       Impact factor: 3.410

8.  A novel method for sample preparation of fresh lung cancer tissue for proteomics analysis by tumor cell enrichment and removal of blood contaminants.

Authors:  Luigi De Petris; Maria Pernemalm; Göran Elmberger; Per Bergman; Lotta Orre; Rolf Lewensohn; Janne Lehtiö
Journal:  Proteome Sci       Date:  2010-02-26       Impact factor: 2.480

9.  Assessment of two immunodepletion methods: off-target effects and variations in immunodepletion efficiency may confound plasma proteomics.

Authors:  Bhavinkumar B Patel; Carlos A Barrero; Alan Braverman; Phillip D Kim; Kelly A Jones; Dian Er Chen; Russell P Bowler; Salim Merali; Steven G Kelsen; Anthony T Yeung
Journal:  J Proteome Res       Date:  2012-10-29       Impact factor: 4.466

10.  Comparative proteomics study on liver mitochondria of primary biliary cirrhosis mouse model.

Authors:  Guang Song; Chaojun Hu; Huishan Zhu; Xi Li; Liying Zhao; Renfang Zhou; Xuan Zhang; Fengchun Zhang; Lin Wu; Yongzhe Li
Journal:  BMC Gastroenterol       Date:  2013-04-12       Impact factor: 3.067
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