OBJECTIVE: To determine if serum concentrations of testosterone precursors would correlate with intratesticular testosterone (ITT) concentration measured directly by testicular aspiration and allow for a less invasive means of inferring ITT. DESIGN: Controlled clinical study. SETTING:Healthy volunteers in an academic research environment. PATIENT(S): Twenty-nine normal men. INTERVENTION(S): We determined ITT concentration by testicular aspiration before and after treatment in men receiving exogenous T to block endogenous gonadotropin production and randomly assigned to one of four doses of hCG (0, 125 IU, 250 IU, or 500 IU every other day) for 3 weeks. MAIN OUTCOME MEASURE(S): The association between serum 17-hydroxyprogesterone (17OH-P), androstenedione, and DHEA and ITT. RESULT(S): With T administration alone, serum 17OH-P decreased significantly and increased significantly when 500 IU hCG was administered. End-of-treatment ITT strongly correlated with serum 17OH-P. Moreover, serum 17OH-P, but not androstenedione or DHEA, was independently associated with end-of-treatment ITT by multivariate linear regression. CONCLUSION(S): Serum 17OH-P is highly correlated with ITT in gonadotropin-suppressed normal men receiving T and stimulated with hCG. Serum 17OH-P is a surrogate biomarker of ITT and may be useful in research and in men receiving gonadotropin therapy for infertility.
RCT Entities:
OBJECTIVE: To determine if serum concentrations of testosterone precursors would correlate with intratesticular testosterone (ITT) concentration measured directly by testicular aspiration and allow for a less invasive means of inferring ITT. DESIGN: Controlled clinical study. SETTING: Healthy volunteers in an academic research environment. PATIENT(S): Twenty-nine normal men. INTERVENTION(S): We determined ITT concentration by testicular aspiration before and after treatment in men receiving exogenous T to block endogenous gonadotropin production and randomly assigned to one of four doses of hCG (0, 125 IU, 250 IU, or 500 IU every other day) for 3 weeks. MAIN OUTCOME MEASURE(S): The association between serum 17-hydroxyprogesterone (17OH-P), androstenedione, and DHEA and ITT. RESULT(S): With T administration alone, serum 17OH-P decreased significantly and increased significantly when 500 IU hCG was administered. End-of-treatment ITT strongly correlated with serum 17OH-P. Moreover, serum 17OH-P, but not androstenedione or DHEA, was independently associated with end-of-treatment ITT by multivariate linear regression. CONCLUSION(S): Serum 17OH-P is highly correlated with ITT in gonadotropin-suppressed normal men receiving T and stimulated with hCG. Serum 17OH-P is a surrogate biomarker of ITT and may be useful in research and in men receiving gonadotropin therapy for infertility.
Authors: Andrea D Coviello; William J Bremner; Alvin M Matsumoto; Karen L Herbst; John K Amory; Bradley D Anawalt; Xiaohua Yan; Terry R Brown; William W Wright; Barry R Zirkin; Jonathan P Jarow Journal: J Androl Date: 2004 Nov-Dec
Authors: M Y Roth; S T Page; K Lin; B D Anawalt; A M Matsumoto; C N Snyder; B T Marck; W J Bremner; J K Amory Journal: J Clin Endocrinol Metab Date: 2010-05-19 Impact factor: 5.958
Authors: Mara Y Roth; Kat Lin; Katrine Bay; John K Amory; Bradley D Anawalt; Alvin M Matsumoto; Brett T Marck; William J Bremner; Stephanie T Page Journal: Fertil Steril Date: 2012-10-03 Impact factor: 7.329
Authors: Andrey A Bakhtyukov; Kira V Derkach; Viktor N Sorokoumov; Anna M Stepochkina; Irina V Romanova; Irina Yu Morina; Irina O Zakharova; Liubov V Bayunova; Alexander O Shpakov Journal: Int J Mol Sci Date: 2021-12-24 Impact factor: 5.923