J K Cooper1, K Ladhani, P D Minor. 1. CJD Resource Centre, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK. jcooper@nibsc.ac.uk
Abstract
BACKGROUND AND OBJECTIVES: A standard panel of materials is needed for the evaluation of assays being developed for the diagnosis of variant Creutzfeldt-Jakob disease. MATERIALS AND METHODS: Tissues from human and animals incubating transmissible spongiform encephalopathy disease have been prepared, aliquoted and where possible characterized by in vitro methods. RESULTS: A standardized preparation of materials has been generated. CONCLUSIONS: Large-scale preparations of tissues and blood fractions can be used to directly compare the sensitivities of assays using different formats.
BACKGROUND AND OBJECTIVES: A standard panel of materials is needed for the evaluation of assays being developed for the diagnosis of variant Creutzfeldt-Jakob disease. MATERIALS AND METHODS: Tissues from human and animals incubating transmissible spongiform encephalopathy disease have been prepared, aliquoted and where possible characterized by in vitro methods. RESULTS: A standardized preparation of materials has been generated. CONCLUSIONS: Large-scale preparations of tissues and blood fractions can be used to directly compare the sensitivities of assays using different formats.
Authors: Matthew T Bishop; Diane L Ritchie; Robert G Will; James W Ironside; Mark W Head; Val Thomson; Moira Bruce; Jean C Manson Journal: PLoS One Date: 2008-08-06 Impact factor: 3.240