Literature DB >> 17451440

Directed evolution of trimethoprim resistance in Escherichia coli.

Morgan Watson1, Jian-Wei Liu, David Ollis.   

Abstract

Directed evolution is a useful tool in the study of enzymes. It is used in this study to investigate the means by which resistance to the antibiotic trimethoprim develops in dihyrofolate reductase from Escherichia coli. Mutants with clinical levels of resistance were obtained after only three generations. After four generations of directed evolution, several mutants were characterized, along with some point mutants made to investigate amino acid changes of interest. Several mutations were found to grant resistance to trimethoprim, both by reducing the binding affinity of the enzyme for the drug, and by increasing the activity of the enzyme.

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Year:  2007        PMID： 17451440     DOI： 10.1111/j.1742-4658.2007.05801.x

Source DB:  PubMed          Journal:  FEBS J        ISSN： 1742-464X            Impact factor:   5.542


  20 in total

Review 1.  Chemical space of Escherichia coli dihydrofolate reductase inhibitors: New approaches for discovering novel drugs for old bugs.

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Journal:  Med Res Rev       Date:  2018-09-07       Impact factor: 12.944

2.  Pathogenic Nocardia cyriacigeorgica and Nocardia nova Evolve To Resist Trimethoprim-Sulfamethoxazole by both Expected and Unexpected Pathways.

Authors:  H Mehta; J Weng; A Prater; R A L Elworth; X Han; Y Shamoo
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3.  Targeted mutations of Bacillus anthracis dihydrofolate reductase condense complex structure−activity relationships.

Authors:  Jennifer M Beierlein; Nanda G Karri; Amy C Anderson
Journal:  J Med Chem       Date:  2010-10-28       Impact factor: 7.446

4.  Direct detection of structurally resolved dynamics in a multiconformation receptor-ligand complex.

Authors:  Mary J Carroll; Anna V Gromova; Keith R Miller; Hao Tang; Xiang Simon Wang; Ashutosh Tripathy; Scott F Singleton; Edward J Collins; Andrew L Lee
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Review 5.  Revitalizing antifolates through understanding mechanisms that govern susceptibility and resistance.

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Journal:  Medchemcomm       Date:  2019-05-08       Impact factor: 3.597

6.  Evolutionary paths to antibiotic resistance under dynamically sustained drug selection.

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7.  Trimethoprim resistance of dihydrofolate reductase variants from clinical isolates of Pneumocystis jirovecii.

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8.  Reduced susceptibility of Moritella profunda dihydrofolate reductase to trimethoprim is not due to glutamate 28.

Authors:  E Joel Loveridge; William M Dawson; Rhiannon M Evans; Anna Sobolewska; Rudolf K Allemann
Journal:  Protein J       Date:  2011-12       Impact factor: 2.371

9.  Rational Design of Novel Allosteric Dihydrofolate Reductase Inhibitors Showing Antibacterial Effects on Drug-Resistant Escherichia coli Escape Variants.

Authors:  Bharath Srinivasan; João V Rodrigues; Sam Tonddast-Navaei; Eugene Shakhnovich; Jeffrey Skolnick
Journal:  ACS Chem Biol       Date:  2017-05-31       Impact factor: 5.100

10.  A multiple antibiotic and serum resistant oligotrophic strain, Klebsiella pneumoniae MB45 having novel dfrA30, is sensitive to ZnO QDs.

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Journal:  Ann Clin Microbiol Antimicrob       Date:  2011-05-19       Impact factor: 3.944
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