BACKGROUND: The green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) possesses anti-carcinogenic properties and was found to induce terminal differentiation in epidermal keratinocytes. Caspase-14, a member of the caspase family associated with epithelial cell differentiation, planned cell death, and barrier formation, is induced by EGCG in normal human epidermal keratinocytes but not in cancer cells. MATERIALS AND METHODS: A human epidermoid cancer cell line, A431, was co-transfected with a caspase-14-expressing pCMV vector and a GFP/neo-etpressingpCMVvector. Cell growth and tumorigenicity of the stable transfectant were determined in comparison to cells transfected with the control GFP/neo-expressing pCMV vector. RESULTS: Expression of exogenous caspase-14 led to growth inhibition and reduced the tumorigenicity of A431 cells. CONCLUSION: Pending future studies, caspase-14 could be used as a novel approach to skin cancer therapy via gene delivery systems.
BACKGROUND: The green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) possesses anti-carcinogenic properties and was found to induce terminal differentiation in epidermal keratinocytes. Caspase-14, a member of the caspase family associated with epithelial cell differentiation, planned cell death, and barrier formation, is induced by EGCG in normal human epidermal keratinocytes but not in cancer cells. MATERIALS AND METHODS: A humanepidermoid cancer cell line, A431, was co-transfected with a caspase-14-expressing pCMV vector and a GFP/neo-etpressingpCMVvector. Cell growth and tumorigenicity of the stable transfectant were determined in comparison to cells transfected with the control GFP/neo-expressing pCMV vector. RESULTS: Expression of exogenous caspase-14 led to growth inhibition and reduced the tumorigenicity of A431 cells. CONCLUSION: Pending future studies, caspase-14 could be used as a novel approach to skin cancer therapy via gene delivery systems.
Authors: Marie-Liesse Asselin-Labat; Kate D Sutherland; François Vaillant; David E Gyorki; Di Wu; Sheridan Holroyd; Kelsey Breslin; Teresa Ward; Wei Shi; Mary L Bath; Siddhartha Deb; Stephen B Fox; Gordon K Smyth; Geoffrey J Lindeman; Jane E Visvader Journal: Mol Cell Biol Date: 2011-09-19 Impact factor: 4.272
Authors: Mengjie Wu; Isamu Kodani; Douglas Dickinson; Frank Huff; Kalu U E Ogbureke; Haiyan Qin; Senthil Arun; Rachel Dulebohn; Mohamed Al-Shabrawey; Amany Tawfik; Susan Prater; Jill Lewis; John Wataha; Regina Messer; Stephen Hsu Journal: Anticancer Res Date: 2009-10 Impact factor: 2.480
Authors: Jean Christopher Chamcheu; Farrukh Afaq; Deeba N Syed; Imtiaz A Siddiqui; Vaqar M Adhami; Naghma Khan; Sohinderjit Singh; Brendan T Boylan; Gary S Wood; Hasan Mukhtar Journal: Exp Dermatol Date: 2013-05 Impact factor: 3.960