BACKGROUND: Current therapeutic approaches to salivary gland cancer are often associated with severe disfigurement and loss of glandular function, which are traumatic to the patients. Exploration of novel treatment approaches, such as gene therapy, is needed. MATERIALS AND METHODS: The human salivary gland cancer cell line HSG was transiently transfected with full length human caspase-14 cDNA. Photomicroscopy, BrdU assay, cell counting, MTT assay, and TUNEL assay were applied. To determine the tumorigenicity, tumor volume, tumor pathology and vascularization were analyzed in vivo. RESULTS: Cell growth and viability were inhibited significantly by transient caspase-14 expression. Caspase-14 expression resulted in a significant reduction of tumorigenicity. Importantly, a significant decrease in tumor blood vessel formation was observed. CONCLUSION: Salivary gland cancer cells underwent growth inhibition, cell death, and reduced tumorigenicity in vivo when exogenous caspase-14 was expressed, which could be due, in part, to an inhibitory effect of caspase-14 on tumor vascularization.
BACKGROUND: Current therapeutic approaches to salivary gland cancer are often associated with severe disfigurement and loss of glandular function, which are traumatic to the patients. Exploration of novel treatment approaches, such as gene therapy, is needed. MATERIALS AND METHODS: The humansalivary gland cancer cell line HSG was transiently transfected with full length humancaspase-14 cDNA. Photomicroscopy, BrdU assay, cell counting, MTT assay, and TUNEL assay were applied. To determine the tumorigenicity, tumor volume, tumor pathology and vascularization were analyzed in vivo. RESULTS: Cell growth and viability were inhibited significantly by transient caspase-14 expression. Caspase-14 expression resulted in a significant reduction of tumorigenicity. Importantly, a significant decrease in tumor blood vessel formation was observed. CONCLUSION:Salivary gland cancer cells underwent growth inhibition, cell death, and reduced tumorigenicity in vivo when exogenous caspase-14 was expressed, which could be due, in part, to an inhibitory effect of caspase-14 on tumor vascularization.
Authors: Kevin Gillespie; Isamu Kodani; Douglas P Dickinson; Kalu U E Ogbureke; Amy M Camba; Mengjie Wu; Stephen Looney; Tin-Chun Chu; Haiyan Qin; Frederick Bisch; Mohamed Sharawy; George S Schuster; Stephen D Hsu Journal: Life Sci Date: 2008-09-06 Impact factor: 5.037
Authors: Y Zhang; H Wang; S Toratani; J D Sato; M Kan; W L McKeehan; T Okamoto Journal: Proc Natl Acad Sci U S A Date: 2001-09-18 Impact factor: 11.205
Authors: Michael Rendl; Jozef Ban; Paul Mrass; Christoph Mayer; Barbara Lengauer; Leopold Eckhart; Wim Declerq; Erwin Tschachler Journal: J Invest Dermatol Date: 2002-11 Impact factor: 8.551