| Literature DB >> 17433448 |
Beate Fuchs1, Celestina Schober, Grit Richter, Rosmarie Süss, Jürgen Schiller.
Abstract
Matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly applied to lipids. However, positional acyl chain analysis of lipids by MALDI was so far scarcely described. In this paper, the fragmentation behavior of phosphatidylethanolamine (PE) is investigated by using post-source decay (PSD) MS. In dependence on the investigated adduct, significant differences could be obtained. It will be shown that in particular the negative ion spectra enable the determination of the individual acyl chains as well as their positions (sn-1 or sn-2). Therefore, MALDI-TOF PSD spectra are a real alternative to more sophisticated MS/MS methods.Entities:
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Year: 2007 PMID： 17433448 DOI： 10.1016/j.jbbm.2007.03.001
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X