BACKGROUND: The objective of this study was to investigate the spectrum of inflammatory gene expression in patients with nasal polyposis. METHODS: The cDNA microarray technique was used to identify gene expression in tissue samples from nasal polyps and adjacent inflammatory nasal mucosa of 21 patients with nonallergic nasal polyposis. To validate the microarray analysis, we compared the expression of five genes by reverse transcription-polymerase chain reaction (RT-PCR): tumor necrosis factor, IL-5, IL-9, fibroblast growth factor, and transforming growth factor (TGF)-beta1. RESULTS: We tested 96 different inflammatory genes in our samples. Thirty-six genes exhibited differences in expression between the two tissue types. In all 36 genes the level of expression was greater in the inflammatory mucosa than the polyps. The RT-PCR confirmed the cDNA results. CONCLUSION: We believe that the high expression of TGF-beta1 in inflammatory mucosa compared with the low expression in polyps may reflect an important role in the inhibitory mechanisms of nasal polyposis.
BACKGROUND: The objective of this study was to investigate the spectrum of inflammatory gene expression in patients with nasal polyposis. METHODS: The cDNA microarray technique was used to identify gene expression in tissue samples from nasal polyps and adjacent inflammatory nasal mucosa of 21 patients with nonallergic nasal polyposis. To validate the microarray analysis, we compared the expression of five genes by reverse transcription-polymerase chain reaction (RT-PCR): tumornecrosis factor, IL-5, IL-9, fibroblast growth factor, and transforming growth factor (TGF)-beta1. RESULTS: We tested 96 different inflammatory genes in our samples. Thirty-six genes exhibited differences in expression between the two tissue types. In all 36 genes the level of expression was greater in the inflammatory mucosa than the polyps. The RT-PCR confirmed the cDNA results. CONCLUSION: We believe that the high expression of TGF-beta1 in inflammatory mucosa compared with the low expression in polyps may reflect an important role in the inhibitory mechanisms of nasal polyposis.
Authors: Spencer C Payne; Joseph K Han; Phillip Huyett; Julie Negri; Elizabeth Z Kropf; Larry Borish; John W Steinke Journal: Am J Rhinol Date: 2008 Nov-Dec
Authors: Leonardo Balsalobre; Rogério Pezato; Claudina Perez-Novo; Maria Teresa S Alves; Rodrigo P Santos; Claus Bachert; Luc L M Weckx Journal: J Otolaryngol Head Neck Surg Date: 2013-04-15
Authors: Rogério Pezato; Leonardo Balsalobre; Milena Lima; Thiago F P Bezerra; Richard L Voegels; Luis Carlos Gregório; Aldo Cassol Stamm; Thibaut van Zele Journal: J Otolaryngol Head Neck Surg Date: 2013-03-28