Jing Li1, Xiang Liu1, Min Sha2, Yong Li1. 1. Department of Otolaryngology, Affiliated Hangzhou First People's Hospital, School of Medicine, Zhejiang University Hangzhou, Zhejiang Province, China. 2. Department of Otolaryngology, Hangzhou Children's Hospital Hangzhou, Zhejiang Province, China.
Abstract
OBJECTIVE: The remodeling patterns in different types of chronic rhinosinusitis (CRS) are controversial. This study aimed to investigate the roles of transforming growth factor-β1 (TGF-β1) and hepatocyte growth factor (HGF) in the CRS remodeling process. METHODS: Surgical samples were obtained from CRSwNP patients (n=29), CRSsNP patients (n=34), and controls (n=21). Collagen deposition was detected via Masson trichrome (MT) staining. Immunohistochemical staining was performed to examine the protein expression of α-smooth muscle actin (α-SMA). The expression of TGF-β1 and HGF was measured by ELISA. The relationship between the rate of TGF-β1/HGF and the expression of Collagen III and α-SMA was analyzed using a Pearson correlation test. Primary nasal epithelial cells (NECs) were cultured and divided into four groups. Collagen III secretion was measured in the supernatants by ELISA. The expression of α-SMA was studied by immunofluorescence. RESULTS: Reduced collagen deposition and α-SMA expression were detected in the CRSwNP group (P=0.033). The expression of collagen deposition and α-SMA was increased in the CRSwNP group (P=0.001). The ELISA tests indicated that TGF-β1 levels were significantly increased in the CRSsNP compared with the controls. The expression of HGF was higher in the CRSwNP than in the other two groups. The ratio of TGF-β1/HGF was upregulated in CRSsNP and was correlated positively with collagen and α-SMA expression (P<0.05, R=0.762). TGF-β1 can increase collagen and α-SMA expression in NECs, and HGF can antagonize the remodeling action of TGF-β1. CONCLUSION: Distinct remodeling patterns are revealed for different types of CRS. The balance of TGF-β1 and HGF is important in the CRS remodeling process. IJCEP
OBJECTIVE: The remodeling patterns in different types of chronic rhinosinusitis (CRS) are controversial. This study aimed to investigate the roles of transforming growth factor-β1 (TGF-β1) and hepatocyte growth factor (HGF) in the CRS remodeling process. METHODS: Surgical samples were obtained from CRSwNP patients (n=29), CRSsNP patients (n=34), and controls (n=21). Collagen deposition was detected via Masson trichrome (MT) staining. Immunohistochemical staining was performed to examine the protein expression of α-smooth muscle actin (α-SMA). The expression of TGF-β1 and HGF was measured by ELISA. The relationship between the rate of TGF-β1/HGF and the expression of Collagen III and α-SMA was analyzed using a Pearson correlation test. Primary nasal epithelial cells (NECs) were cultured and divided into four groups. Collagen III secretion was measured in the supernatants by ELISA. The expression of α-SMA was studied by immunofluorescence. RESULTS: Reduced collagen deposition and α-SMA expression were detected in the CRSwNP group (P=0.033). The expression of collagen deposition and α-SMA was increased in the CRSwNP group (P=0.001). The ELISA tests indicated that TGF-β1 levels were significantly increased in the CRSsNP compared with the controls. The expression of HGF was higher in the CRSwNP than in the other two groups. The ratio of TGF-β1/HGF was upregulated in CRSsNP and was correlated positively with collagen and α-SMA expression (P<0.05, R=0.762). TGF-β1 can increase collagen and α-SMA expression in NECs, and HGF can antagonize the remodeling action of TGF-β1. CONCLUSION: Distinct remodeling patterns are revealed for different types of CRS. The balance of TGF-β1 and HGF is important in the CRS remodeling process. IJCEP
Authors: Ho Sang Rho; Sang Hag Lee; Heung-Man Lee; Seung Hoon Lee; Hak Hyun Jung; Joon Choi; Moo Kyun Park; Sun Mook Kang Journal: Arch Otolaryngol Head Neck Surg Date: 2006-09
Authors: Stewart C Little; S Brandon Early; Charles R Woodard; David C Shonka; Joseph K Han; Larry Borish; John W Steinke Journal: Laryngoscope Date: 2008-02 Impact factor: 3.325
Authors: Nicholas Van Bruaene; Claudina Angela Pérez-Novo; Tomasz M Basinski; Thibaut Van Zele; Gabriele Holtappels; Natalie De Ruyck; Carsten Schmidt-Weber; Cezmi Akdis; Paul Van Cauwenberge; Claus Bachert; Philippe Gevaert Journal: J Allergy Clin Immunol Date: 2008-06 Impact factor: 10.793
Authors: Young H Lee; Yuichiro J Suzuki; Autumn J Griffin; Regina M Day Journal: Am J Physiol Lung Cell Mol Physiol Date: 2008-02-01 Impact factor: 5.464