Literature DB >> 17406584

In vivo imaging of single axons in the mouse spinal cord.

Thomas Misgeld1, Ivana Nikic, Martin Kerschensteiner.   

Abstract

We provide a protocol that describes imaging of single fluorescently labeled axons in the spinal cord of living mice. This method takes advantage of transgenic mouse lines in which the thy1-promoter drives the expression of variants of the green fluorescent protein in a small percentage (less than 1%) of sensory neurons. As a consequence, single axons can be resolved in the surgically exposed dorsal column using wide-field epifluorescence microscopy. This approach allows direct observation of axonal degeneration and regeneration in mouse models of spinal cord pathology for several hours or repetitively over the course of several days.

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Year:  2007        PMID： 17406584     DOI： 10.1038/nprot.2007.24

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  29 in total

1.  Imaging of rat optic nerve axons in vivo.

Authors:  Jan C Koch; Johanna Knöferle; Lars Tönges; Uwe Michel; Mathias Bähr; Paul Lingor
Journal:  Nat Protoc       Date:  2011-11-03       Impact factor: 13.491

2.  Effects of imaging conditions on mitochondrial transport and length in larval motor axons of Drosophila.

Authors:  Kathryn Louie; Gary J Russo; David B Salkoff; Andrea Wellington; Konrad E Zinsmaier
Journal:  Comp Biochem Physiol A Mol Integr Physiol       Date:  2008-06-27       Impact factor: 2.320

3.  Modification of aminosilanized superparamagnetic nanoparticles: feasibility of multimodal detection using 3T MRI, small animal PET, and fluorescence imaging.

Authors:  Lars Stelter; Jens G Pinkernelle; Roger Michel; Ruth Schwartländer; Nathanael Raschzok; Mehmet H Morgul; Martin Koch; Timm Denecke; Juri Ruf; Hans Bäumler; Andreas Jordan; Bernd Hamm; Igor M Sauer; Ulf Teichgräber
Journal:  Mol Imaging Biol       Date:  2009-07-07       Impact factor: 3.488

4.  Near-infrared branding efficiently correlates light and electron microscopy.

Authors:  Derron Bishop; Ivana Nikić; Mary Brinkoetter; Sharmon Knecht; Stephanie Potz; Martin Kerschensteiner; Thomas Misgeld
Journal:  Nat Methods       Date:  2011-06-05       Impact factor: 28.547

5.  Cellular, subcellular and functional in vivo labeling of the spinal cord using vital dyes.

Authors:  Elisa Romanelli; Catherine D Sorbara; Ivana Nikić; Athanasios Dagkalis; Thomas Misgeld; Martin Kerschensteiner
Journal:  Nat Protoc       Date:  2013-02-07       Impact factor: 13.491

6.  Live imaging of astrocyte responses to acute injury reveals selective juxtavascular proliferation.

Authors:  Sophia Bardehle; Martin Krüger; Felix Buggenthin; Julia Schwausch; Jovica Ninkovic; Hans Clevers; Hugo J Snippert; Fabian J Theis; Melanie Meyer-Luehmann; Ingo Bechmann; Leda Dimou; Magdalena Götz
Journal:  Nat Neurosci       Date:  2013-03-31       Impact factor: 24.884

7.  Single-cell axotomy of cultured hippocampal neurons integrated in neuronal circuits.

Authors:  Susana Gomis-Rüth; Michael Stiess; Corette J Wierenga; Liane Meyn; Frank Bradke
Journal:  Nat Protoc       Date:  2014-04-03       Impact factor: 13.491

8.  A method to investigate radial glia cell behavior using two-photon time-lapse microscopy in an ex vivo model of spinal cord development.

Authors:  Janelle M P Pakan; Kieran W McDermott
Journal:  Front Neuroanat       Date:  2014-04-10       Impact factor: 3.856

Review 9.  Axon regeneration after spinal cord injury: insight from genetically modified mouse models.

Authors:  Jae K Lee; Binhai Zheng
Journal:  Restor Neurol Neurosci       Date:  2008       Impact factor: 2.406

10.  Origin, fate and dynamics of macrophages at central nervous system interfaces.

Authors:  Tobias Goldmann; Peter Wieghofer; Marta Joana Costa Jordão; Fabiola Prutek; Nora Hagemeyer; Kathrin Frenzel; Lukas Amann; Ori Staszewski; Katrin Kierdorf; Martin Krueger; Giuseppe Locatelli; Hannah Hochgerner; Robert Zeiser; Slava Epelman; Frederic Geissmann; Josef Priller; Fabio M V Rossi; Ingo Bechmann; Martin Kerschensteiner; Sten Linnarsson; Steffen Jung; Marco Prinz
Journal:  Nat Immunol       Date:  2016-05-02       Impact factor: 25.606
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