Literature DB >> 17406502

Use of a commercially available reagent for the selective detection of homocysteine in plasma.

Jorge O Escobedo1, Weihua Wang, Robert M Strongin.   

Abstract

A procedure for the detection of homocysteine (Hcy) in blood plasma is described. A commercially available chromogen is added to the plasma sample. The plasma solution turns from yellow to blue upon heating for 4 min when a detectable threshold level of Hcy is present. Chromatographic separations and immunogenic materials are not needed. The protocol takes approximately 30 min.

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Year:  2006        PMID： 17406502      PMCID： PMC2662351          DOI： 10.1038/nprot.2006.399

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  13 in total

1.  Evaluation of the AxSYM homocysteine assay and comparison with the IMx homocysteine assay.

Authors:  P Pernet; E Lasnier; M Vaubourdolle
Journal:  Clin Chem       Date:  2000-09       Impact factor: 8.327

2.  Analytical determination of homocysteine: a review.

Authors:  Olga Nekrassova; Nathan S Lawrence; Richard G Compton
Journal:  Talanta       Date:  2003-08-29       Impact factor: 6.057

3.  High-performance liquid chromatographic method for measuring total plasma homocysteine levels.

Authors:  I Fermo; C Arcelloni; G Mazzola; A D'Angelo; R Paroni
Journal:  J Chromatogr B Biomed Sci Appl       Date:  1998-11-20

4.  Plasma homocysteine as a risk factor for dementia and Alzheimer's disease.

Authors:  Sudha Seshadri; Alexa Beiser; Jacob Selhub; Paul F Jacques; Irwin H Rosenberg; Ralph B D'Agostino; Peter W F Wilson; Philip A Wolf
Journal:  N Engl J Med       Date:  2002-02-14       Impact factor: 91.245

5.  N-(1-pyrene)maleimide: a fluorescent cross-linking reagent.

Authors:  C W Wu; L R Yarbrough
Journal:  Biochemistry       Date:  1976-06-29       Impact factor: 3.162

6.  Detection of homocysteine and cysteine.

Authors:  Weihua Wang; Oleksandr Rusin; Xiangyang Xu; Kyu Kwang Kim; Jorge O Escobedo; Sayo O Fakayode; Kristin A Fletcher; Mark Lowry; Corin M Schowalter; Candace M Lawrence; Frank R Fronczek; Isiah M Warner; Robert M Strongin
Journal:  J Am Chem Soc       Date:  2005-11-16       Impact factor: 15.419

7.  Qualitative and quantitative determination of biologically active low-molecular-mass thiols in human blood by reversed-phase high-performance liquid chromatography with photometry and fluorescence detection.

Authors:  A R Ivanov; I V Nazimov; L A Baratova
Journal:  J Chromatogr A       Date:  2000-02-18       Impact factor: 4.759

Review 8.  Homocysteine and cardiovascular disease.

Authors:  H Refsum; P M Ueland; O Nygård; S E Vollset
Journal:  Annu Rev Med       Date:  1998       Impact factor: 13.739

Review 9.  Homocysteine: is it a clinically important cardiovascular risk factor?

Authors:  Steven R Lentz; William G Haynes
Journal:  Cleve Clin J Med       Date:  2004-09       Impact factor: 2.321

Review 10.  Derivatization of thiol-containing compounds.

Authors:  K Shimada; K Mitamura
Journal:  J Chromatogr B Biomed Appl       Date:  1994-09-23
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  8 in total

1.  Fluorescein Tri-Aldehyde Promotes the Selective Detection of Homocysteine.

Authors:  Aabha Barve; Mark Lowry; Jorge O Escobedo; Josephrajan Thainashmuthu; Robert M Strongin
Journal:  J Fluoresc       Date:  2016-01-16       Impact factor: 2.217

Review 2.  Fluorescent Probes with Multiple Binding Sites for the Discrimination of Cys, Hcy, and GSH.

Authors:  Cai-Xia Yin; Kang-Ming Xiong; Fang-Jun Huo; James C Salamanca; Robert M Strongin
Journal:  Angew Chem Int Ed Engl       Date:  2017-09-22       Impact factor: 15.336

3.  Homocystamides promote free-radical and oxidative damage to proteins.

Authors:  Martha Sibrian-Vazquez; Jorge O Escobedo; Soojin Lim; George K Samoei; Robert M Strongin
Journal:  Proc Natl Acad Sci U S A       Date:  2009-12-18       Impact factor: 11.205

4.  Differences in heterocycle basicity distinguish homocysteine from cysteine using aldehyde-bearing fluorophores.

Authors:  Aabha Barve; Mark Lowry; Jorge O Escobedo; Katherine T Huynh; Lovemore Hakuna; Robert M Strongin
Journal:  Chem Commun (Camb)       Date:  2014-08-04       Impact factor: 6.222

5.  A photochemical method for determining plasma homocysteine with limited sample processing.

Authors:  Lovemore Hakuna; Jorge O Escobedo; Mark Lowry; Aabha Barve; Naneki McCallum; Robert M Strongin
Journal:  Chem Commun (Camb)       Date:  2014-03-21       Impact factor: 6.222

6.  Gold nanoparticle sensor for homocysteine thiolactone-induced protein modification.

Authors:  Arther T Gates; Sayo O Fakayode; Mark Lowry; Gabriela M Ganea; Abitha Murugeshu; James W Robinson; Robert M Strongin; Isiah M Warner
Journal:  Langmuir       Date:  2008-03-07       Impact factor: 3.882

7.  Lysosome-Targeting Fluorescence Sensor for Sequential Detection and Imaging of Cu2+ and Homocysteine in Living Cells.

Authors:  Lihua Liu; Hongfei Duan; Haohui Wang; Jieru Miao; Zhihui Wu; Chenxi Li; Yan Lu
Journal:  ACS Omega       Date:  2022-09-14

Review 8.  Thiol reactive probes and chemosensors.

Authors:  Hanjing Peng; Weixuan Chen; Yunfeng Cheng; Lovemore Hakuna; Robert Strongin; Binghe Wang
Journal:  Sensors (Basel)       Date:  2012-11-19       Impact factor: 3.576
  8 in total

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