| Literature DB >> 16277539 |
Weihua Wang1, Oleksandr Rusin, Xiangyang Xu, Kyu Kwang Kim, Jorge O Escobedo, Sayo O Fakayode, Kristin A Fletcher, Mark Lowry, Corin M Schowalter, Candace M Lawrence, Frank R Fronczek, Isiah M Warner, Robert M Strongin.
Abstract
At elevated levels, homocysteine (Hcy, 1) is a risk factor for cardiovascular diseases, Alzheimer's disease, neural tube defects, and osteoporosis. Both 1 and cysteine (Cys, 3) are linked to neurotoxicity. The biochemical mechanisms by which 1 and 3 are involved in disease states are relatively unclear. Herein, we describe simple methods for detecting either Hcy or Cys in the visible spectral region with the highest selectivity reported to date without using biochemical techniques or preparative separations. Simple methods and readily available reagents allow for the detection of Cys and Hcy in the range of their physiologically relevant levels. New HPLC postcolumn detection methods for biological thiols are reported. The potential biomedical relevance of the chemical mechanisms involved in the detection of 1 is described.Entities:
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Year: 2005 PMID： 16277539 PMCID： PMC3386615 DOI： 10.1021/ja054962n
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419