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Literature DB >> 17397

The interaction of N-acetylhexosaminidase with insolubilized concanavalin A.

M G Brattain, P M Kimball, T G Pretlow, M E Marks.

Abstract

The specific interaction between human N-acetylhexosaminidase and concanavalin A was evaluated with respect to temperature, time, pH and concentration of specific ligand in incubation mixtures containing the enzyme and insolubilized lectin. Elution of the enzyme from insolubilized concanavalin A is dependent on both temperature and concentration of alpha-methyl mannoside. Conditions for a high yield of the enzyme from chromatography on insolubilized concanavalin A are described.

Entities: Chemical Species

Mesh：

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Year: 1977 PMID： 17397 PMCID： PMC1164690 DOI： 10.1042/bj1630247

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

13 in total

1. The purification of carcinoembryonic antigen by glutaraldehyde cross-linked concanavalin A.

Authors: M G Brattain; C M Jones; J M Pittman; T G Pretlow
Journal: Biochem Biophys Res Commun Date: 1975-07-08 Impact factor: 3.575

2. The isolation of carcinoembryonic antigen from tumor tissue at neutral pH.

Authors: R J Carrico; M Usategui-Gomez
Journal: Cancer Res Date: 1975-11 Impact factor: 12.701

3. A recognition marker required for uptake of a lysosomal enzyme by cultured fibroblasts.

Authors: S Hickman; L J Shapiro; E F Neufeld
Journal: Biochem Biophys Res Commun Date: 1974-03-15 Impact factor: 3.575

4. Determination of protein: a modification of the Lowry method that gives a linear photometric response.

Authors: E F Hartree
Journal: Anal Biochem Date: 1972-08 Impact factor: 3.365

5. Binding of human liver beta-galactosidases to plant lectins insolubilized on agarose.

Authors: A G Norden; J S O'Brien
Journal: Biochem Biophys Res Commun Date: 1974-01 Impact factor: 3.575

6. ConA-sepharose affinity chromatography: a study on the glycoprotein nature of brain acid hydrolases.

Authors: S Bishayee; B K Bachhawat
Journal: Neurobiology Date: 1974

7. Binding of human fibroblast interferon to concanavalin A-agarose. Involvement of carbohydrate recognition and hydrophobic interaction.

Authors: M W Davey; E Sulkowski; W A Carter
Journal: Biochemistry Date: 1976-02-10 Impact factor: 3.162

8. Introduction of purified hexosaminidase A into Tay-Sachs leukocytes by means of immunoglobulin-coated liposomes.

Authors: C M Cohen; G Weissmann; S Hoffstein; Y C Awasthi; S K Srivastava
Journal: Biochemistry Date: 1976-01-27 Impact factor: 3.162

9. Demonstration of two molecular variants of carcinoembryonic antigen by concanavalin A sepharose affinity chromatography.

Authors: S R Harvey; T M Chu
Journal: Cancer Res Date: 1975-11 Impact factor: 12.701

10. N-Acetyl-beta-glucosaminidases in human spleen.

Authors: D Robinson; J L Stirling
Journal: Biochem J Date: 1968-04 Impact factor: 3.857

8 in total

1. Kinetics and thermodynamic transitions of N-acetyl-beta-D-glucosaminidase A and B in free and bound forms: role of cellulose ion-exchangers.

Authors: G S Gupta; R Raina
Journal: Mol Cell Biochem Date: 1989-03-16 Impact factor: 3.396

The interaction of N-acetylhexosaminidase with insolubilized concanavalin A.

1. The purification of carcinoembryonic antigen by glutaraldehyde cross-linked concanavalin A.

2. The isolation of carcinoembryonic antigen from tumor tissue at neutral pH.

3. A recognition marker required for uptake of a lysosomal enzyme by cultured fibroblasts.

4. Determination of protein: a modification of the Lowry method that gives a linear photometric response.

5. Binding of human liver beta-galactosidases to plant lectins insolubilized on agarose.

6. ConA-sepharose affinity chromatography: a study on the glycoprotein nature of brain acid hydrolases.

7. Binding of human fibroblast interferon to concanavalin A-agarose. Involvement of carbohydrate recognition and hydrophobic interaction.

8. Introduction of purified hexosaminidase A into Tay-Sachs leukocytes by means of immunoglobulin-coated liposomes.

9. Demonstration of two molecular variants of carcinoembryonic antigen by concanavalin A sepharose affinity chromatography.

10. N-Acetyl-beta-glucosaminidases in human spleen.

1. Kinetics and thermodynamic transitions of N-acetyl-beta-D-glucosaminidase A and B in free and bound forms: role of cellulose ion-exchangers.

Review 2. Binding interactions of glycoproteins with lectins.

3. Characterization of an unusual isoenzyme of N-acetyl-beta-D-hexosaminidase from a human colonic carcinoma cell line.

4. Purification and some properties of tartrate-sensitive acid phosphatase from rabbit kidney cortex.

5. Heparin-sepharose affinity chromatography for purification of bull seminal-plasma hyaluronidase.

6. Isolation of beta-N-acetylhexosaminidase from rabbit semen and its role in fertilization.

7. Clearance of human N-acetyl-beta-hexosaminidases from rat circulation.

8. Purification, biochemical properties and active sites of N-acetyl-beta-D-hexosaminidases from human seminal plasma.