| Literature DB >> 17383817 |
Gopal Dhar1, Smita Mehta, Snigdha Banerjee, Ashleigh Gardner, Bryan M McCarty, Sharad C Mathur, Donald R Campbell, Suman Kambhampati, Sushanta K Banerjee.
Abstract
The objective of this study was to explore the pathophysiological relevance of WISP-2/CCN5 in progression of human pancreatic adenocarcinoma (PAC). We found WISP-2/CCN5 mRNA and protein expression was faint and sporadic in PAC and detected in only 8.7-20% of the samples with varying grades as compared to adjacent normal and chronic pancreatitis samples where expression was very high in the ducts and acini. Colocalization studies in tissue-microarray slides revealed WISP-2/CCN5 mRNA loss was associated with p53 overexpression in PAC. Like tissue samples, p53 mutant-PAC cell lines show loss of WISP-2/CCN5. Moreover, functional analysis studies demonstrate exposure of pancreatic cancer cells to WISP-2/CCN5 recombinant protein enhances mesenchymal-epithelial-transition (MET). Collectively, we suggest WISP-2/CCN5 silencing may be a critical event during differentiation and progression of PAC and mutant p53 is possibly an important player in pursuing this episode.Entities:
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Year: 2007 PMID： 17383817 DOI： 10.1016/j.canlet.2007.02.012
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679