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Literature DB >> 1738200

Functional characterization of a U5 ribozyme: intracellular suppression of human immunodeficiency virus type 1 expression.

B Dropulić¹, N H Lin, M A Martin, K T Jeang.

Abstract

We have designed a ribozyme that cleaves human immunodeficiency virus type 1 (HIV-1) RNA in U5 (at nucleotide +115). This ribozyme was tested in vitro and was found to give efficient and specific digestion of RNA containing the HIV-1 U5 sequence. When the U5 ribozyme was placed into the HIV-1 genome, virus replication was suppressed in tissue culture. Introduction of this ribozyme into cells by using an amphotropic retrovirus vector significantly reduced expression of U5-containing RNA in cells chronically infected with HIV-1. Naive T cells were cocultivated with packaging cells that produce defective amphotropic retroviruses containing the U5 ribozyme. These lymphocytes were found to be partially protected from HIV-1 infection.

Entities: Disease Species

Mesh：

Substances：

Year: 1992 PMID： 1738200 PMCID： PMC240867

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

35 in total

1. Importance of the nef gene for maintenance of high virus loads and for development of AIDS.

Authors: H W Kestler; D J Ringler; K Mori; D L Panicali; P K Sehgal; M D Daniel; R C Desrosiers
Journal: Cell Date: 1991-05-17 Impact factor: 41.582

2. Can ribozymes be used to regulate procaryote gene expression?

Authors: J C Chuat; F Galibert
Journal: Biochem Biophys Res Commun Date: 1989-08-15 Impact factor: 3.575

3. Autolytic processing of dimeric plant virus satellite RNA.

Authors: G A Prody; J T Bakos; J M Buzayan; I R Schneider; G Bruening
Journal: Science Date: 1986-03-28 Impact factor: 47.728

4. The accuracy of reverse transcriptase from HIV-1.

Authors: J D Roberts; K Bebenek; T A Kunkel
Journal: Science Date: 1988-11-25 Impact factor: 47.728

5. A small catalytic oligoribonucleotide.

Authors: O C Uhlenbeck
Journal: Nature Date: 1987 Aug 13-19 Impact factor: 49.962

6. A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors: F L Graham; A J van der Eb
Journal: Virology Date: 1973-04 Impact factor: 3.616

7. Expression of human glucocerebrosidase in long-term reconstituted mice following retroviral-mediated gene transfer into hematopoietic stem cells.

Authors: P H Correll; Y Kew; L K Perry; R O Brady; J K Fink; S Karlsson
Journal: Hum Gene Ther Date: 1990 Impact factor: 5.695

8. Specific gene suppression by engineered ribozymes in monkey cells.

Authors: F H Cameron; P A Jennings
Journal: Proc Natl Acad Sci U S A Date: 1989-12 Impact factor: 11.205

9. Inhibition of human immunodeficiency virus type 1 replication in human T cells stably expressing antisense RNA.

Authors: G Sczakiel; M Pawlita
Journal: J Virol Date: 1991-01 Impact factor: 5.103

10. A safe packaging line for gene transfer: separating viral genes on two different plasmids.

Authors: D Markowitz; S Goff; A Bank
Journal: J Virol Date: 1988-04 Impact factor: 5.103

37 in total

1. Inhibition of hepatitis B virus X gene expression by novel DNA enzymes.

Authors: R Goila; A C Banerjea
Journal: Biochem J Date: 2001-02-01 Impact factor: 3.857

2. HIV-1 LTR as a target for synthetic ribozyme-mediated inhibition of gene expression: site selection and inhibition in cell culture.

Authors: B Bramlage; E Luzi; F Eckstein
Journal: Nucleic Acids Res Date: 2000-11-01 Impact factor: 16.971

Functional characterization of a U5 ribozyme: intracellular suppression of human immunodeficiency virus type 1 expression.

1. Importance of the nef gene for maintenance of high virus loads and for development of AIDS.

2. Can ribozymes be used to regulate procaryote gene expression?

3. Autolytic processing of dimeric plant virus satellite RNA.

4. The accuracy of reverse transcriptase from HIV-1.

5. A small catalytic oligoribonucleotide.

6. A new technique for the assay of infectivity of human adenovirus 5 DNA.

7. Expression of human glucocerebrosidase in long-term reconstituted mice following retroviral-mediated gene transfer into hematopoietic stem cells.

8. Specific gene suppression by engineered ribozymes in monkey cells.

9. Inhibition of human immunodeficiency virus type 1 replication in human T cells stably expressing antisense RNA.

10. A safe packaging line for gene transfer: separating viral genes on two different plasmids.

1. Inhibition of hepatitis B virus X gene expression by novel DNA enzymes.

2. HIV-1 LTR as a target for synthetic ribozyme-mediated inhibition of gene expression: site selection and inhibition in cell culture.

3. Ribozymes that cleave reovirus genome segment S1 also protect cells from pathogenesis caused by reovirus infection.

4. Expression of lexA targeted ribozyme in Escherichia coli BL-21 (DE3) cells.

5. Can hammerhead ribozymes be efficient tools to inactivate gene function?

Review 6. Antiviral therapy for human immunodeficiency virus infections.

Review 7. Antigene, ribozyme and aptamer nucleic acid drugs: progress and prospects.

8. Effective ribozyme delivery in plant cells.

9. Ribozyme mediated degradation of beta-amyloid peptide precursor mRNA in COS-7 cells.

10. A hairpin ribozyme inhibits expression of diverse strains of human immunodeficiency virus type 1.