Literature DB >> 17369836

Multiplexed protein detection by proximity ligation for cancer biomarker validation.

Simon Fredriksson1, William Dixon, Hanlee Ji, Albert C Koong, Michael Mindrinos, Ronald W Davis.   

Abstract

We present a proximity ligation-based multiplexed protein detection procedure in which several selected proteins can be detected via unique nucleic-acid identifiers and subsequently quantified by real-time PCR. The assay requires a 1-microl sample, has low-femtomolar sensitivity as well as five-log linear range and allows for modular multiplexing without cross-reactivity. The procedure can use a single polyclonal antibody batch for each target protein, simplifying affinity-reagent creation for new biomarker candidates.

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Year:  2007        PMID： 17369836     DOI： 10.1038/nmeth1020

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  70 in total

1.  The maternal plasma proteome changes as a function of gestational age in normal pregnancy: a longitudinal study.

Authors:  Roberto Romero; Offer Erez; Eli Maymon; Piya Chaemsaithong; Zhonghui Xu; Percy Pacora; Tinnakorn Chaiworapongsa; Bogdan Done; Sonia S Hassan; Adi L Tarca
Journal:  Am J Obstet Gynecol       Date:  2017-03-03       Impact factor: 8.661

2.  Inntags: small self-structured epitopes for innocuous protein tagging.

Authors:  Maya V Georgieva; Galal Yahya; Laia Codó; Raúl Ortiz; Laura Teixidó; José Claros; Ricardo Jara; Mònica Jara; Antoni Iborra; Josep Lluís Gelpí; Carme Gallego; Modesto Orozco; Martí Aldea
Journal:  Nat Methods       Date:  2015-08-31       Impact factor: 28.547

Review 3.  Nanotechnology and cancer.

Authors:  James R Heath; Mark E Davis
Journal:  Annu Rev Med       Date:  2008       Impact factor: 13.739

Review 4.  Single-cell sequencing-based technologies will revolutionize whole-organism science.

Authors:  Ehud Shapiro; Tamir Biezuner; Sten Linnarsson
Journal:  Nat Rev Genet       Date:  2013-07-30       Impact factor: 53.242

5.  Sensitive plasma protein analysis by microparticle-based proximity ligation assays.

Authors:  Spyros Darmanis; Rachel Yuan Nong; Maria Hammond; Jijuan Gu; Anders Alderborn; Johan Vänelid; Agneta Siegbahn; Sigrun Gustafsdottir; Olle Ericsson; Ulf Landegren; Masood Kamali-Moghaddam
Journal:  Mol Cell Proteomics       Date:  2009-11-27       Impact factor: 5.911

6.  Multiplexed homogeneous proximity ligation assays for high-throughput protein biomarker research in serological material.

Authors:  Martin Lundberg; Stine Buch Thorsen; Erika Assarsson; Andrea Villablanca; Bonnie Tran; Nick Gee; Mick Knowles; Birgitte Sander Nielsen; Eduardo González Couto; Roberto Martin; Olle Nilsson; Christian Fermer; Jörg Schlingemann; Ib Jarle Christensen; Hans-Jorgen Nielsen; Björn Ekström; Claes Andersson; Mats Gustafsson; Nils Brunner; Jan Stenvang; Simon Fredriksson
Journal:  Mol Cell Proteomics       Date:  2011-01-17       Impact factor: 5.911

7.  Western blotting via proximity ligation for high performance protein analysis.

Authors:  Yanling Liu; Jijuan Gu; Åsa Hagner-McWhirter; Poojahrau Sathiyanarayanan; Mats Gullberg; Ola Söderberg; Johan Johansson; Maria Hammond; Daniel Ivansson; Ulf Landegren
Journal:  Mol Cell Proteomics       Date:  2011-08-02       Impact factor: 5.911

Review 8.  Aptamers and the next generation of diagnostic reagents.

Authors:  Varatharasa Thiviyanathan; David G Gorenstein
Journal:  Proteomics Clin Appl       Date:  2012-12       Impact factor: 3.494

9.  Photocleavable DNA barcode-antibody conjugates allow sensitive and multiplexed protein analysis in single cells.

Authors:  Sarit S Agasti; Monty Liong; Vanessa M Peterson; Hakho Lee; Ralph Weissleder
Journal:  J Am Chem Soc       Date:  2012-11-02       Impact factor: 15.419

10.  Identification of a biomarker panel using a multiplex proximity ligation assay improves accuracy of pancreatic cancer diagnosis.

Authors:  Stephanie T Chang; Jacob M Zahn; Joe Horecka; Pamela L Kunz; James M Ford; George A Fisher; Quynh T Le; Daniel T Chang; Hanlee Ji; Albert C Koong
Journal:  J Transl Med       Date:  2009-12-11       Impact factor: 5.531
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