| Literature DB >> 17360444 |
Masaru Matsuda1, Ai Shinomiya, Masato Kinoshita, Aya Suzuki, Tohru Kobayashi, Bindhu Paul-Prasanth, En-lieng Lau, Satoshi Hamaguchi, Mitsuru Sakaizumi, Yoshitaka Nagahama.
Abstract
Although the sex-determining gene SRY/Sry has been identified in mammals, homologues and genes that have a similar function have yet to be identified in nonmammalian vertebrates. Recently, DMY (the DM-domain gene on the Y chromosome) was cloned from the sex-determining region on the Y chromosome of the teleost fish medaka (Oryzias latipes). DMY has been shown to be required for the normal development of male individuals. In this study, we show that a 117-kb genomic DNA fragment that carries DMY is able to induce testis differentiation and subsequent male development in XX (genetically female) medaka. In addition, overexpression of DMY cDNA under the control of the CMV promoter also caused XX sex reversal. These results demonstrate that DMY is sufficient for male development in medaka and suggest that the functional difference between the X and Y chromosomes in medaka is a single gene. Our data indicate that DMY is an additional sex-determining gene in vertebrates.Entities:
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Year: 2007 PMID： 17360444 PMCID： PMC1820675 DOI： 10.1073/pnas.0611707104
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205