Literature DB >> 17357500

Immunomagnetic indirect positive sorting of precartilaginous stem cells from neonatal rat.

Hao Cheng1, Anmin Chen, Hongbo You.   

Abstract

To investigate the technique of sorting high-purity precartilaginous stem cells from rat's perichondrium, neonatal rat's perichondrium cells suspensions were incubated with monoclone antibody of anti-fibroblast growth factor receptor-3 (anti-FGFR-3), and the labeled cells were separated from the suspension in the magnetic field by immuno-beads coated with the second antibody. Purity of the sorted neural stem cells was found to be 93.0%-99.0%, with living cells amounting to 80% -85 %. The magnetic cell sorting system could effectively separate precartilaginous stem cells from perichondrium cell suspensions.

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Year:  2006        PMID： 17357500     DOI： 10.1007/s11596-006-0626-0

Source DB:  PubMed          Journal:  J Huazhong Univ Sci Technolog Med Sci        ISSN： 1672-0733


  5 in total

1.  A new method for histamine release from purified peripheral blood basophils using monoclonal antibody-coated magnetic beads.

Authors:  H Nishi; S Nishimura; M Higashiura; N Ikeya; H Ohta; T Tsuji; M Nishimura; S Ohnishi; H Higashi
Journal:  J Immunol Methods       Date:  2000-06-23       Impact factor: 2.303

2.  High gradient magnetic cell separation with MACS.

Authors:  S Miltenyi; W Müller; W Weichel; A Radbruch
Journal:  Cytometry       Date:  1990

3.  Fibroblast growth factor receptor-3 as a marker for precartilaginous stem cells.

Authors:  D Robinson; A Hasharoni; N Cohen; A Yayon; R M Moskowitz; Z Nevo
Journal:  Clin Orthop Relat Res       Date:  1999-10       Impact factor: 4.176

Review 4.  Magnetic separation techniques: their application to medicine.

Authors:  J T Kemshead; J Ugelstad
Journal:  Mol Cell Biochem       Date:  1985-05       Impact factor: 3.396

5.  Detection of prostatic specific membrane antigen messenger RNA using immunobead reverse transcriptase polymerase chain reaction.

Authors:  R A Ghossein; I Osman; S Bhattacharya; J Ferrara; M Fazzari; C Cordon-Cardo; H I Scher
Journal:  Diagn Mol Pathol       Date:  1999-06
  5 in total
  1 in total

1.  Chondrogenic effect of cell-based scaffold of self-assembling peptides/PLGA-PLL loading the hTGFβ3 plasmid DNA.

Authors:  Qiyong Pan; Wenkai Li; Xuefeng Yuan; Yeltay Rakhmanov; Pengcheng Wang; Rui Lu; Zekai Mao; Xiaobin Shang; Hongbo You
Journal:  J Mater Sci Mater Med       Date:  2015-12-16       Impact factor: 3.896
  1 in total

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