| Literature DB >> 17349634 |
Hitoshi Hiura1, Junichi Komiyama, Motomu Shirai, Yayoi Obata, Hidehiko Ogawa, Tomohiro Kono.
Abstract
Mouse genomes show a large cluster of imprinted genes at the Dlk1-Gtl2 domain in the distal region of chromosome 12. An intergenic-differentially methylated region (IG-DMR) located between Dlk1 and Gtl2 is specifically methylated in the male germline; IG-DMR regulates the parental allele-specific expression of imprinted genes. Here, we show the resetting of IG-DMR methylation marks during male germ-cell differentiation. For parental allele-specific methylation analysis, polymorphisms were detected in a 2.6-kb IG-DMR in three mouse strains. Bisulfite methylation analysis showed erasure of the marks by E14 and re-establishment before birth. The IG-DMR methylation status was maintained in spermatogonia and spermatocytes of mature testes. The IG-DMR methylation status established before birth is thus maintained throughout the lifetime in the male germline.Entities:
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Year: 2007 PMID： 17349634 DOI： 10.1016/j.febslet.2007.02.034
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124