OBJECTIVE: Preeclampsia and small-for-gestational age (SGA) neonates have partially overlapping clinicopathologic features. MicroRNAs (miRNAs) are critical posttranscriptional regulators of gene expression. This study was performed to determine whether preeclampsia and SGA are associated with alterations in placental miRNA expression. STUDY DESIGN: Placentas were obtained from patients with (1) preeclampsia (n = 9); (2) SGA (n = 9); (3) preeclampsia + SGA (n = 9); and (4) a control group with spontaneous preterm labor and delivery (PTL; n = 9). The expression of 157 miRNAs was assessed by real-time quantitative reverse transcription-polymerase chain reaction. RESULTS: Differential expression between preeclampsia and the control group (miR-210, miR-182) and between preeclampsia + SGA and the control group (miR-210, miR-182*, and others) was found. Gene Ontology analysis of the target genes revealed enrichment for specific biological process categories (antiapoptosis: miR-182; regulation of transcription: miR-210). CONCLUSION: This study reports, for the first time, increased expression of specific placental miRNAs in preeclampsia with and without SGA. The findings also provide novel targets for further investigation of the pathophysiology of preeclampsia.
OBJECTIVE: Preeclampsia and small-for-gestational age (SGA) neonates have partially overlapping clinicopathologic features. MicroRNAs (miRNAs) are critical posttranscriptional regulators of gene expression. This study was performed to determine whether preeclampsia and SGA are associated with alterations in placental miRNA expression. STUDY DESIGN: Placentas were obtained from patients with (1) preeclampsia (n = 9); (2) SGA (n = 9); (3) preeclampsia + SGA (n = 9); and (4) a control group with spontaneous preterm labor and delivery (PTL; n = 9). The expression of 157 miRNAs was assessed by real-time quantitative reverse transcription-polymerase chain reaction. RESULTS: Differential expression between preeclampsia and the control group (miR-210, miR-182) and between preeclampsia + SGA and the control group (miR-210, miR-182*, and others) was found. Gene Ontology analysis of the target genes revealed enrichment for specific biological process categories (antiapoptosis: miR-182; regulation of transcription: miR-210). CONCLUSION: This study reports, for the first time, increased expression of specific placental miRNAs in preeclampsia with and without SGA. The findings also provide novel targets for further investigation of the pathophysiology of preeclampsia.
Authors: Matthew A Maccani; Michele Avissar-Whiting; Carolyn E Banister; Bethany McGonnigal; James F Padbury; Carmen J Marsit Journal: Epigenetics Date: 2010-10-01 Impact factor: 4.528
Authors: Daniel A Enquobahrie; Mark Hensley; Chunfang Qiu; Dejene F Abetew; Karin Hevner; Mahlet G Tadesse; Michelle A Williams Journal: Reprod Sci Date: 2015-10-27 Impact factor: 3.060
Authors: Katie L Davis-Anderson; Sebastian Berger; Emilie R Lunde-Young; Vishal D Naik; Heewon Seo; Greg A Johnson; Hanno Steen; Jayanth Ramadoss Journal: Alcohol Clin Exp Res Date: 2017-08-09 Impact factor: 3.455