Literature DB >> 17341123

Synergetic effects of nanoporous support and urea on enzyme activity.

Chenghong Lei1, Yongsoon Shin, Jun Liu, Eric J Ackerman.   

Abstract

We report synergetic effects of functionalized mesoporous silica (FMS) and urea to promote favorable protein conformational changes. The specific activity of glucose isomerase (GI) entrapped in FMS in the presence of urea was approximately double that of GI in solution in the absence of urea. Rather than losing all activity in a denaturing solution of 8.0 M urea, the specific activity of GI entrapped in FMS remained higher than the highest specific activity of GI free in solution.

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Year:  2007        PMID: 17341123     DOI: 10.1021/nl070255b

Source DB:  PubMed          Journal:  Nano Lett        ISSN: 1530-6984            Impact factor:   11.189


  7 in total

1.  Enzymatic conversion of CO(2) to bicarbonate in functionalized mesoporous silica.

Authors:  Yuehua Yu; Baowei Chen; Wen Qi; Xiaolin Li; Yongsoon Shin; Chenghong Lei; Jun Liu
Journal:  Microporous Mesoporous Mater       Date:  2012-05-12       Impact factor: 5.455

Review 2.  Macromolecular crowding and confinement: biochemical, biophysical, and potential physiological consequences.

Authors:  Huan-Xiang Zhou; Germán Rivas; Allen P Minton
Journal:  Annu Rev Biophys       Date:  2008       Impact factor: 12.981

3.  Probing mechanisms for enzymatic activity enhancement of organophosphorus hydrolase in functionalized mesoporous silica.

Authors:  Baowei Chen; Chenghong Lei; Yongsoon Shin; Jun Liu
Journal:  Biochem Biophys Res Commun       Date:  2009-10-27       Impact factor: 3.575

4.  Functionalized nanoporous silica for the removal of heavy metals from biological systems: adsorption and application.

Authors:  Wassana Yantasee; Ryan D Rutledge; Wilaiwan Chouyyok; Vichaya Sukwarotwat; Galya Orr; Cynthia L Warner; Marvin G Warner; Glen E Fryxell; Robert J Wiacek; Charles Timchalk; R Shane Addleman
Journal:  ACS Appl Mater Interfaces       Date:  2010-10       Impact factor: 9.229

5.  Covalent anchoring of chloroperoxidase and glucose oxidase on the mesoporous molecular sieve SBA-15.

Authors:  Dirk Jung; Carsten Streb; Martin Hartmann
Journal:  Int J Mol Sci       Date:  2010-02-24       Impact factor: 5.923

6.  The role of nonbonded interactions in the conformational dynamics of organophosphorous hydrolase adsorbed onto functionalized mesoporous silica surfaces.

Authors:  Diego E B Gomes; Roberto D Lins; Pedro G Pascutti; Chenghong Lei; Thereza A Soares
Journal:  J Phys Chem B       Date:  2010-01-14       Impact factor: 2.991

7.  Thin-layer polymer wrapped enzymes encapsulated in hierarchically mesoporous silica with high activity and enhanced stability.

Authors:  Fang Zhang; Meitao Wang; Chao Liang; Huangyong Jiang; Jian Shen; Hexing Li
Journal:  Sci Rep       Date:  2014-03-21       Impact factor: 4.379

  7 in total

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