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Literature DB >> 17336929

Identification of a novel PP2C-type mitochondrial phosphatase.

Mandar Joshi¹, Nam Ho Jeoung, Kirill M Popov, Robert A Harris.

Abstract

A novel phosphatase has been cloned and partially characterized. It has a mitochondrial leader sequence and its amino acid sequence places it in the PP2C family like two known mitochondrial phosphatases. Western blot analysis of subcellular fractions and confocal microscopy of 3T3L1 preadipocytes expressing the GFP-tagged protein confirm its mitochondrial localization. Western blot analysis indicates that the protein is expressed in several mouse tissues, with highest expression in brain, heart, liver, and kidney. The recombinant protein exhibits Mn(2+)-dependent phosphoserine phosphatase activity against the branched-chain alpha-keto acid dehydrogenase complex, suggesting the enzyme may play a role in regulation of branched chain amino acid catabolism. Whether there are other mitochondrial substrates for the enzyme is not known.

Entities: Gene Species

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Year: 2007 PMID： 17336929 PMCID： PMC1876681 DOI： 10.1016/j.bbrc.2007.02.108

Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575

24 in total

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