Literature DB >> 17332645

Real-time PCR fluorescent chemistries.

John Mackay1, Olfert Landt.   

Abstract

There are more than a dozen formats available for the fluorescent detection of amplified DNA in kinetic (real-time) PCR. These chemistries are adaptable to most real-time PCR instruments and may offer benefits over the usual manufacturer-recommended chemistries for the instrument. The most popular chemistries are the generic dye, SYBR Green I, TaqMan, and hybridization probes. However, there are now new dyes being reported with superior fluorescent detection and product resolution; as well as new probe formats that may offer advanced multiplexing opportunities for quantification and genotyping.

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Year:  2007        PMID： 17332645     DOI： 10.1385/1-59745-229-7:237

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

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2.  Preparation and Use of Cellular Reagents: A Low-resource Molecular Biology Reagent Platform.

Authors:  Sanchita Bhadra; Inyup Paik; Jose-Angel Torres; Stéphane Fadanka; Chiara Gandini; Harry Akligoh; Jenny Molloy; Andrew D Ellington
Journal:  Curr Protoc       Date:  2022-03

3.  Active liquid crystals powered by force-sensing DNA-motor clusters.

Authors:  Alexandra M Tayar; Michael F Hagan; Zvonimir Dogic
Journal:  Proc Natl Acad Sci U S A       Date:  2021-07-27       Impact factor: 11.205

Review 4.  Field effect sensors for nucleic Acid detection: recent advances and future perspectives.

Authors:  Bruno Veigas; Elvira Fortunato; Pedro V Baptista
Journal:  Sensors (Basel)       Date:  2015-05-04       Impact factor: 3.576

5.  Characterization of transcription factor phenotypes within antigen-specific CD4+ T cells using qualitative multiplex single-cell RT-PCR.

Authors:  Chansavath Phetsouphanh; Yin Xu; Janaki Amin; Nabila Seddiki; Francesco Procopio; Rafick Pierre Sekaly; John J Zaunders; Anthony D Kelleher
Journal:  PLoS One       Date:  2013-10-04       Impact factor: 3.240

Review 6.  Listeria monocytogenes in foods-From culture identification to whole-genome characteristics.

Authors:  Jacek Osek; Beata Lachtara; Kinga Wieczorek
Journal:  Food Sci Nutr       Date:  2022-05-03       Impact factor: 3.553

7.  Single-Tubed Wild-Type Blocking Quantitative PCR Detection Assay for the Sensitive Detection of Codon 12 and 13 KRAS Mutations.

Authors:  Jun-Fu Huang; Dong-Zhu Zeng; Guang-Jie Duan; Yan Shi; Guo-Hong Deng; Han Xia; Han-Qing Xu; Na Zhao; Wei-Ling Fu; Qing Huang
Journal:  PLoS One       Date:  2015-12-23       Impact factor: 3.240

8.  Enhanced specificity of TPMT*2 genotyping using unidirectional wild-type and mutant allele-specific scorpion primers in a single tube.

Authors:  Dong Chen; Zhao Yang; Han Xia; Jun-Fu Huang; Yang Zhang; Tian-Nun Jiang; Gui-Yu Wang; Zheng-Ran Chuai; Wei-Ling Fu; Qing Huang
Journal:  PLoS One       Date:  2014-04-04       Impact factor: 3.240
  8 in total

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