Literature DB >> 17329809

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 from Mycobacterium tuberculosis.

Georgios N Hatzopoulos1, Jochen Mueller-Dieckmann.   

Abstract

Initiation factor 1 (IF-1; Rv3462c) from Mycobacterium tuberculosis, a component of the 30S initiation complex, was cloned and heterologously expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and crystallized. A complete data set has been collected to high resolution. The crystals belonged to space group P2(1)2(1)2, with two molecules per asymmetric unit which are related by translational symmetry.

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Year:  2007        PMID： 17329809      PMCID： PMC2330178          DOI： 10.1107/S174430910700437X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  9 in total

1.  SFCHECK: a unified set of procedures for evaluating the quality of macromolecular structure-factor data and their agreement with the atomic model.

Authors:  A A Vaguine; J Richelle; S J Wodak
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-01-01

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Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-05-29

3.  The CCP4 suite: programs for protein crystallography.

Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

Review 4.  Initiation of mRNA translation in prokaryotes.

Authors:  C O Gualerzi; C L Pon
Journal:  Biochemistry       Date:  1990-06-26       Impact factor: 3.162

5.  The open-access high-throughput crystallization facility at EMBL Hamburg.

Authors:  Jochen Mueller-Dieckmann
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2006-11-23

6.  'Touchdown' PCR to circumvent spurious priming during gene amplification.

Authors:  R H Don; P T Cox; B J Wainwright; K Baker; J S Mattick
Journal:  Nucleic Acids Res       Date:  1991-07-25       Impact factor: 16.971

7.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

8.  Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.

Authors:  S T Cole; R Brosch; J Parkhill; T Garnier; C Churcher; D Harris; S V Gordon; K Eiglmeier; S Gas; C E Barry; F Tekaia; K Badcock; D Basham; D Brown; T Chillingworth; R Connor; R Davies; K Devlin; T Feltwell; S Gentles; N Hamlin; S Holroyd; T Hornsby; K Jagels; A Krogh; J McLean; S Moule; L Murphy; K Oliver; J Osborne; M A Quail; M A Rajandream; J Rogers; S Rutter; K Seeger; J Skelton; R Squares; S Squares; J E Sulston; K Taylor; S Whitehead; B G Barrell
Journal:  Nature       Date:  1998-06-11       Impact factor: 49.962

Review 9.  The TB structural genomics consortium: a resource for Mycobacterium tuberculosis biology.

Authors:  T C Terwilliger; M S Park; G S Waldo; J Berendzen; L-W Hung; C-Y Kim; C V Smith; J C Sacchettini; M Bellinzoni; R Bossi; E De Rossi; A Mattevi; A Milano; G Riccardi; M Rizzi; M M Roberts; A R Coker; G Fossati; P Mascagni; A R M Coates; S P Wood; C W Goulding; M I Apostol; D H Anderson; H S Gill; D S Eisenberg; B Taneja; S Mande; E Pohl; V Lamzin; P Tucker; M Wilmanns; C Colovos; W Meyer-Klaucke; A W Munro; K J McLean; K R Marshall; D Leys; J K Yang; H-J Yoon; B I Lee; M G Lee; J E Kwak; B W Han; J Y Lee; S-H Baek; S W Suh; M M Komen; V L Arcus; E N Baker; J S Lott; W Jacobs; T Alber; B Rupp
Journal:  Tuberculosis (Edinb)       Date:  2003       Impact factor: 3.131
  9 in total

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